Difference between revisions of "Team:Goettingen/Results"
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<a href="" onClick=" $('#menu1').slideToggle(300, function callback() { }); return false;"><h1> Competent Cell Test Kit, RFP construct (iGEM)</h1></a> | <a href="" onClick=" $('#menu1').slideToggle(300, function callback() { }); return false;"><h1> Competent Cell Test Kit, RFP construct (iGEM)</h1></a> | ||
Revision as of 10:22, 15 September 2015
Project Results
Competent Cell Test Kit, RFP construct (iGEM)
Before using our competent E. coli TOP10 cells in the important experiments, we used the Competent Cell Test Kit to test the efficiency of our competent cells!
The kit includes five vials of each different DNA concentration: 50pg/μl, 20pg/μl, 10pg/μl, 5pg/μl, 0.5pg/μl of purified DNA from BBa_J04450 (RFP construct) in plasmid backbone pSB1C3.
The first test transformation (1 μl plasmid used, 20 μl plated) showed very poor results:
|
DNA concentration |
0.5pg/μl |
5pg/μl |
10pg/μl |
20pg/μl |
50pg/μl |
|
# of colonies |
0 |
0 |
2 |
0 |
13 |
|
efficiency (cfu) |
0 |
0 |
3.8x106 |
0 |
3.4x106 |
|
Results |
efficiency |
|
average |
1.4x106 |
|
weighted |
3.5x106 |
Here you can describe the results of your project and your future plans.
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- Clearly and objectively describe the results of your work.
- Future plans for the project
- Considerations for replicating the experiments
Project Achievements
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- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.