Difference between revisions of "Team:NYU Shanghai/Protocols"
Line 30: | Line 30: | ||
margin: 0px; | margin: 0px; | ||
padding: 0px; | padding: 0px; | ||
− | font-size: | + | font-size: 20px; |
overflow: visible | overflow: visible | ||
} | } | ||
Line 210: | Line 210: | ||
<h3>Protocols</h3> | <h3>Protocols</h3> | ||
<h6>We built our constructs from digests and ligations of pre-made biobrick parts. Our general conclusion is that 3A assembly is generally inefficient, and an insufficient method for adding small parts (such as a terminator) to a larger construction within pSB1C3. We learned that ratios were extremely important in the process of 3A Assembly, and we made a summary sheet of the equations we used in pre-digest and pre-ligation that accounts for digest dilution and amount needed to ensure results are seen on a gel, not just ligation ratios. </h6> | <h6>We built our constructs from digests and ligations of pre-made biobrick parts. Our general conclusion is that 3A assembly is generally inefficient, and an insufficient method for adding small parts (such as a terminator) to a larger construction within pSB1C3. We learned that ratios were extremely important in the process of 3A Assembly, and we made a summary sheet of the equations we used in pre-digest and pre-ligation that accounts for digest dilution and amount needed to ensure results are seen on a gel, not just ligation ratios. </h6> | ||
+ | <br> | ||
</div> | </div> | ||
Revision as of 12:35, 15 September 2015