Difference between revisions of "Team:Goettingen/Experiments"
Ashaikhqasem (Talk | contribs) |
Ashaikhqasem (Talk | contribs) |
||
Line 22: | Line 22: | ||
} | } | ||
− | #menu1, #menu2, #menu3, #menu4, #menu5, #menu6, #menu7, #menu8, #menu9, #menu10, #menu11, #menu12, #menu13, #menu14, #menu15, #menu16, #menu17, #menu18, #menu19, #menu20, #menu21, #menu22, #menu23, #menu24{ | + | #menu1, #menu2, #menu3, #menu4, #menu5, #menu6, #menu7, #menu8, #menu9, #menu10, #menu11, #menu12, #menu13, #menu14, #menu15, #menu16, #menu17, #menu18, #menu19, #menu20, #menu21, #menu22, #menu23, #menu24, #menu25, #menu26{ |
display:none; | display:none; | ||
width:90%; | width:90%; | ||
Line 1,563: | Line 1,563: | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
+ | </div> | ||
+ | |||
+ | <h2> Sequencing </h2> | ||
+ | <a href="" onClick=" $('#menu25').slideToggle(300, function callback() { }); return false;"><h1> Protocol for Sanger sequencing</h1></a> | ||
+ | <div id="menu25"> | ||
+ | <p align="center"><strong>Protocol for Sanger sequencing</strong></p> | ||
+ | <ul> | ||
+ | <li>Sequencing reaction setup</li> | ||
+ | </ul> | ||
+ | <table style="width: 541px;" border="1" cellspacing="0" cellpadding="0" align="left"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td valign="top" width="205"> | ||
+ | <p align="center"><strong>Component</strong></p> | ||
+ | </td> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center"><strong>PCR product</strong></p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center"><strong>Template quantity</strong></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td rowspan="8" valign="top" width="205"> | ||
+ | <p align="center"> </p> | ||
+ | <p align="center"> </p> | ||
+ | <p align="center"> </p> | ||
+ | <p align="center">DNA</p> | ||
+ | </td> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">100 - 200 bp</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">3 – 7 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">200 - 500 bp</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">7 – 12 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">500 - 1000 bp</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">12 – 25 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">1000 - 2000 bp</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">25 – 45 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">>2000 bp</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">45 – 80 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">Double stranded</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">500 - 1500 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">Single stranded</p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">250 - 500 ng</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center"><em>Cosmid, BAC</em></p> | ||
+ | </td> | ||
+ | <td valign="top" width="162"> | ||
+ | <p align="center">2 – 4 µg</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="205"> | ||
+ | <p>Forward primer (5pmol/ µl )</p> | ||
+ | </td> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">1 µl</p> | ||
+ | </td> | ||
+ | <td rowspan="4" valign="top" width="162"> | ||
+ | <p align="center"> </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="205"> | ||
+ | <p>Reverse primer (5pmol/ µl )</p> | ||
+ | </td> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">1 µl</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="205"> | ||
+ | <p>Nuclease free H<sub>2</sub>O</p> | ||
+ | </td> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center">XX µl</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td valign="top" width="205"> | ||
+ | <p><strong>Total volume</strong></p> | ||
+ | </td> | ||
+ | <td valign="top" width="174"> | ||
+ | <p align="center"><em>5 </em>µl</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <ul> | ||
+ | <li>Forward and Reverse primers should be added in 2 individual eppendorf tubes.</li> | ||
+ | </ul> | ||
+ | <p></p> | ||
+ | <p> </p> | ||
+ | <p></p> | ||
+ | <p> </p> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <a href="" onClick=" $('#menu26').slideToggle(300, function callback() { }); return false;"> | ||
+ | <h1> Sanger sequencing reaction setup for overnight</h1></a> | ||
+ | <div id="menu26"> | ||
+ | |||
+ | <p align="center"><span style="font-size: x-large; color: #888888;"><strong></strong></span></p> | ||
+ | <p align="center"> </p> | ||
+ | <ul> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"><strong><span style="font-size: 14.0pt; line-height: 115%;">Sanger sequencing reaction setup for overnight</span></strong></p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <table class="MsoNormalTable" style="width: 289px; border-collapse: collapse; margin-left: 6.75pt; margin-right: 6.75pt;" border="1" cellspacing="0" cellpadding="0" align="left"> | ||
+ | <tbody> | ||
+ | <tr style="mso-yfti-irow: 0; mso-yfti-firstrow: yes; height: 17.1pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-bottom: solid windowtext 1.0pt; mso-border-alt: solid black .75pt; mso-border-bottom-alt: solid windowtext .5pt; padding: .75pt .75pt .75pt .75pt; height: 17.1pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><strong><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Components</span></strong></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: solid black 1.0pt; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-left-alt: inset windowtext .75pt; mso-border-top-alt: solid black .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 17.1pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><strong><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Volume</span></strong></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 1; height: 5.0pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 5.0pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Purified Plasmid </span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid black 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: solid windowtext .5pt; mso-border-left-alt: inset windowtext .75pt; mso-border-bottom-alt: solid black .75pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 5.0pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">800ng</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 2; height: 9.75pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: inset windowtext .75pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Forward primer (</span><span style="font-size: 12.0pt;">5pmol/ </span><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">µl )</span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid black 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext; mso-border-left-alt: inset windowtext; mso-border-bottom-alt: solid black; mso-border-right-alt: solid black; mso-border-width-alt: .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">1 µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 3; height: 9.75pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: inset windowtext .75pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Reverse primer (</span><span style="font-size: 12.0pt;">5pmol/ </span><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">µl )</span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid black 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext; mso-border-left-alt: inset windowtext; mso-border-bottom-alt: solid black; mso-border-right-alt: solid black; mso-border-width-alt: .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">1 µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 4; height: 15.75pt;"> | ||
+ | <td style="width: 153.75pt; border-top: none; border-left: solid black 1.0pt; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext .75pt; mso-border-left-alt: solid black .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 15.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Nuclease free H<sub>2</sub>O</span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext .75pt; mso-border-left-alt: inset windowtext .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 15.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">XX µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 5; mso-yfti-lastrow: yes; height: 18.75pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 18.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal"><strong><span style="font-size: 12.0pt; line-height: 115%; mso-bidi-font-family: 'Verdana\,Bold';">Total volume</span></strong></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: solid windowtext .5pt; mso-border-left-alt: inset windowtext .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 18.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt;"><em><span style="font-size: 12.0pt; mso-bidi-font-family: 'Verdana\,Italic';">15 </span></em><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"> </p> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"><strong><span style="font-size: 14.0pt; line-height: 115%;"></span></strong></p> | ||
+ | <p class="MsoListParagraph" style="margin-left: 38.7pt; mso-add-space: auto; text-indent: -.25in; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-size: 12.0pt; line-height: 115%; font-family: Symbol; mso-fareast-font-family: Symbol; mso-bidi-font-family: Symbol;">·<span style="font-stretch: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman';"> </span></span><!--[endif]--><span style="font-size: 12.0pt; line-height: 115%; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Forward and Reverse primers should be added in 2 individual eppendorf tubes.</span></p> | ||
+ | <p class="MsoListParagraph" style="margin-left: 38.7pt; mso-add-space: auto;"><span style="font-size: 12.0pt; line-height: 115%;"> </span></p> | ||
+ | <table class="MsoNormalTable" style="width: 289px; border-collapse: collapse; margin-left: 6.75pt; margin-right: 6.75pt;" border="1" cellspacing="0" cellpadding="0" align="left"> | ||
+ | <tbody> | ||
+ | <tr style="mso-yfti-irow: 0; mso-yfti-firstrow: yes; height: 17.1pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-bottom: solid windowtext 1.0pt; mso-border-alt: solid black .75pt; mso-border-bottom-alt: solid windowtext .5pt; padding: .75pt .75pt .75pt .75pt; height: 17.1pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><strong><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Components</span></strong></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: solid black 1.0pt; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-left-alt: inset windowtext .75pt; mso-border-top-alt: solid black .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 17.1pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><strong><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Volume</span></strong></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 1; height: 5.0pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 5.0pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Purified Plasmid </span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid black 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: solid windowtext .5pt; mso-border-left-alt: inset windowtext .75pt; mso-border-bottom-alt: solid black .75pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 5.0pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">800ng</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 2; height: 9.75pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: inset windowtext .75pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Forward primer (</span><span style="font-size: 12.0pt;">5pmol/ </span><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">µl )</span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid black 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext; mso-border-left-alt: inset windowtext; mso-border-bottom-alt: solid black; mso-border-right-alt: solid black; mso-border-width-alt: .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">1 µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 3; height: 9.75pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: inset windowtext .75pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Reverse primer (</span><span style="font-size: 12.0pt;">5pmol/ </span><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">µl )</span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid black 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext; mso-border-left-alt: inset windowtext; mso-border-bottom-alt: solid black; mso-border-right-alt: solid black; mso-border-width-alt: .75pt; padding: .75pt .75pt .75pt .75pt; height: 9.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">1 µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 4; height: 15.75pt;"> | ||
+ | <td style="width: 153.75pt; border-top: none; border-left: solid black 1.0pt; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext .75pt; mso-border-left-alt: solid black .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 15.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Nuclease free H<sub>2</sub>O</span></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: inset windowtext .75pt; mso-border-left-alt: inset windowtext .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 15.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt; vertical-align: baseline;"><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">XX µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="mso-yfti-irow: 5; mso-yfti-lastrow: yes; height: 18.75pt;"> | ||
+ | <td style="width: 153.75pt; border: solid black 1.0pt; border-top: none; mso-border-top-alt: solid windowtext .5pt; mso-border-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 18.75pt;" valign="top" width="205"> | ||
+ | <p class="MsoNormal"><strong><span style="font-size: 12.0pt; line-height: 115%; mso-bidi-font-family: 'Verdana\,Bold';">Total volume</span></strong></p> | ||
+ | </td> | ||
+ | <td style="width: 63.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid black 1.0pt; mso-border-top-alt: solid windowtext .5pt; mso-border-left-alt: inset windowtext .75pt; mso-border-bottom-alt: solid windowtext .5pt; mso-border-right-alt: solid black .75pt; padding: .75pt .75pt .75pt .75pt; height: 18.75pt;" valign="top" width="84"> | ||
+ | <p class="MsoNormal" style="margin-bottom: 0.0001pt;"><em><span style="font-size: 12.0pt; mso-bidi-font-family: 'Verdana\,Italic';">15 </span></em><span style="font-size: 12.0pt; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">µl</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <p class="MsoNormal" style="text-align: center;" align="center"><strong><span style="font-size: 14.0pt; line-height: 115%;">Sanger sequencing reaction setup for overnight</span></strong></p> | ||
+ | <p class="MsoNormal"><a name="_GoBack"></a> </p> | ||
+ | <p class="MsoNormal"> </p> | ||
+ | <p class="MsoNormal"> </p> | ||
+ | <p class="MsoNormal"> </p> | ||
+ | <p class="MsoNormal"> </p> | ||
+ | <p class="MsoNormal"> </p> | ||
+ | <p class="MsoListParagraph" style="margin-left: 38.7pt; mso-add-space: auto; text-indent: -.25in; mso-list: l0 level1 lfo1;"><!--[if !supportLists]--><span style="font-size: 12.0pt; line-height: 115%; font-family: Symbol; mso-fareast-font-family: Symbol; mso-bidi-font-family: Symbol;">·<span style="font-stretch: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman';"> </span></span><!--[endif]--><span style="font-size: 12.0pt; line-height: 115%; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'Segoe UI';">Forward and Reverse primers should be added in 2 individual eppendorf tubes.</span></p> | ||
+ | <p class="MsoNormal"><span style="font-size: 12.0pt; line-height: 115%;"> </span></p> | ||
</div> | </div> | ||
</html> | </html> |
Revision as of 15:33, 15 September 2015