Difference between revisions of "Team:UMaryland/Results"
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<h1 align = "center">Considerations</h1> | <h1 align = "center">Considerations</h1> | ||
− | + | <p style = "font-size:24px">PCR of Hok-Sok Construct</p> | |
+ | <br> | ||
+ | <p style = "font-size:18px">Amplification of the Hok-Sok cassette is difficult due to the high inherent secondary structure in the construct. Hok ssDNA is capable of naturally folding into a stable secondary structure, resulting in early terminations and other side products. We recommend that a reaction buffer suitable for difficult templates be used, such as Phusion GC buffer + added DMSO.</p> | ||
<h2> Project Results</h2> | <h2> Project Results</h2> | ||
Revision as of 17:53, 15 September 2015