Difference between revisions of "Team:Freiburg/Project/pRIG15 18"
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Link to GeneBank file: <a class="media" href="https://static.igem.org/mediawiki/2015/b/b0/Freiburg_2015_BBa_K1621005.gb" title="2015_Freiburg_BBa_K1621005" src="https://static.igem.org/mediawiki/2015/b/b0/Freiburg_2015_BBa_K1621005.gb">BBa_K1621005.gb</a>. | Link to GeneBank file: <a class="media" href="https://static.igem.org/mediawiki/2015/b/b0/Freiburg_2015_BBa_K1621005.gb" title="2015_Freiburg_BBa_K1621005" src="https://static.igem.org/mediawiki/2015/b/b0/Freiburg_2015_BBa_K1621005.gb">BBa_K1621005.gb</a>. | ||
</br> | </br> | ||
| − | Link to Registry: <a href="http://parts.igem.org/Part: | + | Link to Registry: <a href="http://parts.igem.org/Part:BBa_K1621005" target="_blank">BBa_K1621005</a> |
</p> | </p> | ||
</div> | </div> | ||
Revision as of 16:52, 16 September 2015
pRIG15_18
Treponama pallidum is responsible for the sexually transmitted disease syphillis with over 12 million new cases each year worldwide. 1) For our project we used the sequence of the bacterioferritin protein TpF1, an antigen of Treponema pallidum which reacts specifically with infected human serum. 2) Additionally, this antigen shows strong antibody responses what makes it good target for antigen-antibody interaction studies.3)
To insert the sequence for TpF1 into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR and then assembled with the digested pSB1C3 backbone using Gibson Assembly. To prove correct insertion of the fragment we performed a test digest and verified the part by sequencing. Link to GeneBank file: BBa_K1621005.gb. Link to Registry: BBa_K1621005
