Difference between revisions of "Team:WHU-Pharm/Description"

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<h1> WHU-Pharm Project Description </h1>
 
<h1> WHU-Pharm Project Description </h1>
  
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<h2> Abstract </h2>
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<h2> Background </h2>
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<p> Tumor cells are found to have increased ability to consume glucose due to their high growth rate, thus causing the surrounding glucose concentration to drop. Therefore, it is possible to discriminate tumor cells from other tissue cells by detecting the low glucose concentration. Using liposome as a vector, we hope to design a drug expression system with the ability to respond to different glucose level. To achieve this, we combine the CRP activator, a regulatory protein activated by cAMP, with red fluorescent protein (RPF) as a reporter of expression level. Since glucose concentration is reversely related with cAMP level, a change of glucose level can influence the binding of CRP with target operon, thus altering the fluorescent intensity. By expressing our desinged gene circuit with ''in vitro'' protein expression system, we then test the expression level of the gene and find out how it relates with glucose concentration.</p>
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<p> Tumor cells are characterized by their high growth and poliferation rate. Because of this, they need to consume much more glucose as a source of energy. While blood glucose concentration stays stable under normal circumastances, tissues invaded by tumor cells could show a decreased level of glucose concentration. The difference of glucose concentration provides us a promising way to discriminate tumor cells from normal cells. </p>
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<p>Once low concentration of glucose is detected, it would be good if the “sensor” could take action to destroy the tumor cells. To achieve this, we plan to combine the glucose sensor with a liposome, which contains the enzymes and genes needed to de novo synthesize a certain anti-tumor drug.
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<h4>Advice on writing your Project Description</h4>
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<h2> Glucose Sensor</h2>
  
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h4>References</h4>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
 
  
  
  
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<h4>Inspiration</h4>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
 
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Revision as of 16:05, 17 September 2015

WHU-Pharm Project Description

Background

Tumor cells are characterized by their high growth and poliferation rate. Because of this, they need to consume much more glucose as a source of energy. While blood glucose concentration stays stable under normal circumastances, tissues invaded by tumor cells could show a decreased level of glucose concentration. The difference of glucose concentration provides us a promising way to discriminate tumor cells from normal cells.

Once low concentration of glucose is detected, it would be good if the “sensor” could take action to destroy the tumor cells. To achieve this, we plan to combine the glucose sensor with a liposome, which contains the enzymes and genes needed to de novo synthesize a certain anti-tumor drug.


Glucose Sensor

We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.

Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.