Difference between revisions of "Team:UMBC-Maryland/Notebook"

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<br> [[June 8th]]
 
<br> [[June 8th]]
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<br> Digest products with a combination of restriction enzymes ( XbaI, HindIII, EcoRI)were ran on a 2% agarose gel. Both gel results came up inconclusive.  
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<br> Digest products with a combination of restriction enzymes (XbaI, HindIII, EcoRI)were ran on a 2% agarose gel. Both gel results came up inconclusive.
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<br> [[June 25th]]
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<br> Mini-prep of the metallothionein plasmid.
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<br> [[June 29th]]
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<br> Digestion of the metallothionein plasmid with restriction enzymes (EcoRI & PstI). The digestion products were then run on a gel stained with ethidium bromide.  
 
<p> Document the dates you worked on your project.</p>
 
<p> Document the dates you worked on your project.</p>
  

Revision as of 17:40, 29 June 2015



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Notebook

'''Week 1'''

[[June 1st]]
First meeting of the project over summer where we ran our PCR products on a 1% agarose gel with results shown in 6_1_15PCR.jpg. Band sizzes are determined from the ladder in N0467_thumb.gif.

[[June 4th]]
iGEM team members attended a meeting with a professor and was given an overview to the procedure of 3A Assembly. We also discussed team structure and the CUP1 gene construct that is needed to be synthesized as a G-block. G-blocks are then submitted to IDT for sequencing.

[[June 8th]]
Digest products with a combination of restriction enzymes (XbaI, HindIII, EcoRI)were ran on a 2% agarose gel. Both gel results came up inconclusive.

[[June 25th]]
Mini-prep of the metallothionein plasmid.

[[June 29th]]
Digestion of the metallothionein plasmid with restriction enzymes (EcoRI & PstI). The digestion products were then run on a gel stained with ethidium bromide.

Document the dates you worked on your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.

Inspiration

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