Difference between revisions of "Team:WHU-Pharm/Results"

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<h2> Measurement of Enzyme Concentration
 
<h2> Measurement of Enzyme Concentration
 
<p> We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.
 
<p> We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.
<img src=https://static.igem.org/mediawiki/2015/5/56/WHU-Pharm_EnzymeConcentration.jpeg />
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<img src=https://static.igem.org/mediawiki/2015/thumb/5/56/WHU-Pharm_EnzymeConcentration.jpeg/184px-WHU-Pharm_EnzymeConcentration.jpeg />
  
  

Revision as of 19:12, 17 September 2015

Project Results

Purification of Enzymes

We have successfully purified the enzymes needed. Below is the SDS-PAGE result of most of these enzymes.

From 1 to 10: Glk, Pgi, PfkA, FbaA, TpiA, Pgk, GpmA, Eno, PykF, GapA (M: protein ladder)

Measurement of Enzyme Concentration

We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.

Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

  • A list of linked bullet points of the successful results during your project
  • A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.