Difference between revisions of "Team:WHU-Pharm/Results"

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<p> From 1 to 10: Glk, Pgi, PfkA, FbaA, TpiA, Pgk, GpmA, Eno, PykF, GapA (M: protein ladder)
 
<p> From 1 to 10: Glk, Pgi, PfkA, FbaA, TpiA, Pgk, GpmA, Eno, PykF, GapA (M: protein ladder)
  
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<h2> Measurement of Enzyme Concentration
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<h2> Measurement of Enzyme Concentration</h2>
 
<p> We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.</p>
 
<p> We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.</p>
 
<img src=https://static.igem.org/mediawiki/2015/thumb/5/56/WHU-Pharm_EnzymeConcentration.jpeg/459px-WHU-Pharm_EnzymeConcentration.jpeg />
 
<img src=https://static.igem.org/mediawiki/2015/thumb/5/56/WHU-Pharm_EnzymeConcentration.jpeg/459px-WHU-Pharm_EnzymeConcentration.jpeg />
  
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<h2><i>In Vitro</i> Expression</h2>
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<p>We used protein expression kit by ThermoFisher Scientific &copy; to express part Bba_J04450 along with the purified enzyme. Unfortunately, we failed to acquire a convincing result. This is possibly due to the inappropriate ratio of different components. We will continue to test different settings to test our designed glucose sensor. </p>
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<h4> Project Achievements </h4>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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</ul>
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Revision as of 19:19, 17 September 2015

Project Results

Purification of Enzymes

We have successfully purified the enzymes needed. Below is the SDS-PAGE result of most of these enzymes.

From 1 to 10: Glk, Pgi, PfkA, FbaA, TpiA, Pgk, GpmA, Eno, PykF, GapA (M: protein ladder)

Measurement of Enzyme Concentration

We used Pierce™ Microplate BCA Protein Assay Kit to measure concentrations of each enzyme.

In Vitro Expression

We used protein expression kit by ThermoFisher Scientific © to express part Bba_J04450 along with the purified enzyme. Unfortunately, we failed to acquire a convincing result. This is possibly due to the inappropriate ratio of different components. We will continue to test different settings to test our designed glucose sensor.