Difference between revisions of "Team:Freiburg/Project/Overview"
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<h2>The Concept</h2> | <h2>The Concept</h2> | ||
<p> | <p> | ||
| − | The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis of disease related antigens and a novel method for label-free detection - all this packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams. | + | The key feature of the <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Project/System">DiaCHIP concept</a> is the combination of on-demand protein synthesis of disease related antigens and a novel method for label-free detection - all this packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams. |
</p> | </p> | ||
</div> | </div> | ||
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<div class="slidertext"> | <div class="slidertext"> | ||
<h1>The DiaCHIP - System Overview</h1> | <h1>The DiaCHIP - System Overview</h1> | ||
| − | <p>The core of our new diagnostic device | + | <p>The core of our new diagnostic device consists of two slides that form a microfluidic chamber. Therein, an antigen array can be generated on demand on the bottom slide. By flushing the chamber with a blood sample, antibodies present in the sample bind to corresponding antigens. This interaction is detected in real-time using the optical detection method iRIf.</p> |
<p style="margin-top:30px"> | <p style="margin-top:30px"> | ||
<div class="intro_button menu-arrow"> | <div class="intro_button menu-arrow"> | ||
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<div class="slidertext"> | <div class="slidertext"> | ||
<h1>Outlook</h1> | <h1>Outlook</h1> | ||
| − | <p></p> | + | <p>Our results provide a proof of concept that the functional priciple of the DiaCHIP is suitable for antibody detection in complex samples. Although further improvements have to be done in terms of reliablitiy and quantification, various additional applications are conceivable. Representing a way to reduce time and cost required for testing a single patient, the DiaCHIP holds the potential to enhance and enlighten future diagnostics. |
| − | + | </p> | |
| − | <div class=" | + | <p style="margin-top:30px"> |
| − | + | <div class="intro_button menu-arrow"> | |
| − | + | <a href="https://2015.igem.org/Team:Freiburg/Project/Future_Directions" title="Future_Directions">DiaCHIP in the <br>future</a> | |
</div> | </div> | ||
| + | </p> | ||
</li> | </li> | ||
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<div class="slidertext"> | <div class="slidertext"> | ||
<h1>Surface Chemistry</h1> | <h1>Surface Chemistry</h1> | ||
| − | <p>The production of a customized protein microarray in the DiaCHIP is based on selective immobilization of antigens on a glass slide. Therefore, a specific surface chemistry | + | <p>The production of a customized protein microarray in the DiaCHIP is based on selective immobilization of antigens on a glass slide. Therefore, a specific surface chemistry was established to reduce the proportion of unspecific binding of non-target proteins to a minimum. |
Read more about the different layer compositions we tested on our way to high specificity. | Read more about the different layer compositions we tested on our way to high specificity. | ||
</p> | </p> | ||
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<div class="slidertext"> | <div class="slidertext"> | ||
<h1>Cell-Free Expression</h1> | <h1>Cell-Free Expression</h1> | ||
| − | <p> A key feature of the DiaCHIP is the capability to produce protein arrays on demand via cell-free expression. | + | <p> A key feature of the DiaCHIP is the capability to produce protein arrays on demand via cell-free expression. To reduce the cost of a DiaCHIP measurement, we produced a cell-free expression system based on an <i>E. coli</i> lysate ourselves. See how this sensitive system was established and optimized. |
</p> | </p> | ||
| + | <p style="margin-top:30px"> | ||
| + | <div class="intro_button menu-arrow"> | ||
| + | <a href="https://2015.igem.org/Team:Freiburg/Project/Cellfree_Expression" title="Protein Purification">Cell-free antigen expression</a> | ||
</div> | </div> | ||
| − | + | </p> | |
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<div class="slidertext"> | <div class="slidertext"> | ||
<h1>Diagnostics Today</h1> | <h1>Diagnostics Today</h1> | ||
| − | <p>Currently used serological tests are available for a broad range of infectious diseases. However, they meet limitations that | + | <p>Currently used serological tests are available for a broad range of infectious diseases. However, they meet limitations that restrict an early onset of appropriate treatments which could be life-saving. The necessity of performing several tests to check for more than one disease at once is not only time-consuming but also costly. The DiaCHIP tackles these issues by providing a fast and affordable method for simultaneous testing. |
</p> | </p> | ||
Revision as of 21:44, 17 September 2015
write appropriate content for slider: should present main subpoints of project in short sentence and link to them
add pictures nto slider (ps1709)
The DiaCHIP - A Versatile Detection System
Project Motivation
Serological tests are a key element in modern medicine. Especially for detection and identification of infectious diseases, performing several blood tests is inevitable. Testing for more than one disease at a time or diagnosing a patient with diffuse symptoms usually requires more than one serological test. Every single test that is performed increases the time of waiting for a result as well as the bill. In case of dangerous infectious diseases every minute until the onset of the appropriate treatment is important for life. What if there was a possibility to combine all this testing in one single chip which is affordable for everyone?
Detecting Antigen-Antibody Interactions
The DiaCHIP is an innovative tool to screen for a broad range of antibodies present in serum. Antibodies serve as indicator for an immune response towards an infectious diseases or a successful vaccination. They also play an important role in the diagnosis of autoimmune diseases. Identifying diseases by detecting disease associated antibodies in a patient's serum is an established method in modern diagnostics.
Based on the very same principle, the DiaCHIP enables to simultaneously screen for multiple diseases at time, thereby reducing time and cost of a diagnosis. Especially the ability to differentiate between life threatening diseases and mild infections within a short time bears the potential to save lives.
The Concept
The key feature of the DiaCHIP concept is the combination of on-demand protein synthesis of disease related antigens and a novel method for label-free detection - all this packed into one device. The idea is to overcome challenges commonly found in protein array production and preservation. In addition, results can be obtained in a time- and cost-efficient manner; with a device simple enough to be rebuilt by future iGEM teams.
-
-
Outlook
Our results provide a proof of concept that the functional priciple of the DiaCHIP is suitable for antibody detection in complex samples. Although further improvements have to be done in terms of reliablitiy and quantification, various additional applications are conceivable. Representing a way to reduce time and cost required for testing a single patient, the DiaCHIP holds the potential to enhance and enlighten future diagnostics.
Cell-Free Expression
A key feature of the DiaCHIP is the capability to produce protein arrays on demand via cell-free expression. To reduce the cost of a DiaCHIP measurement, we produced a cell-free expression system based on an E. coli lysate ourselves. See how this sensitive system was established and optimized.
