Difference between revisions of "Team:NYU Shanghai/Protocols"

m
m
Line 313: Line 313:
 
  <div id="lucText" style="display:none">
 
  <div id="lucText" style="display:none">
 
   <br>
 
   <br>
   <h5><font color="white">NYU Shanghai Construct</font></h5>
+
   <h5><font color="white">NYU Shanghai Construct</font>
 
   <br>
 
   <br>
 
   <p><img width="800" src="https://static.igem.org/mediawiki/2015/1/12/NYU_Shanghai_Luciferase_Protein.png">
 
   <p><img width="800" src="https://static.igem.org/mediawiki/2015/1/12/NYU_Shanghai_Luciferase_Protein.png">
 +
  <br>
 +
  <img width="800" src="https://static.igem.org/mediawiki/2015/9/9a/NYU_Shanghai_Chromo_Procedure_2.png">
 
   <br>
 
   <br>
 
     Overview
 
     Overview

Revision as of 00:14, 18 September 2015

Protocols

We built our constructs from pre-made biobrick parts. Our overall conclusion is that 3A assembly is generally inefficient, and an insufficient method for adding small parts (such as a terminator) to a larger construction within pSB1C3. We learned that ratios were extremely important in the process of 3A Assembly, and we made a summary sheet of the equations we used in pre-digest and pre-ligation that accounts for digest dilution and amount needed to ensure results are seen on a gel, not just ligation ratios. We wished we used gibson assembly.


Making Color

Recipes

3A Assembly

Calculations (pdf)