Difference between revisions of "Team:NYU Shanghai/Protocols"

m
Line 472: Line 472:
 
  <div id="chromoText" style="display:none">
 
  <div id="chromoText" style="display:none">
 
     <p>
 
     <p>
     <h6><font color="white" size="24">Building our Construct</font></h6>
+
     <h6><font color="#d66">Building our Construct</font></h6>
 
     <img src="https://static.igem.org/mediawiki/2015/d/df/NYU_Shanghai_Chromo_Procedure.png" width="800">
 
     <img src="https://static.igem.org/mediawiki/2015/d/df/NYU_Shanghai_Chromo_Procedure.png" width="800">
     <br><br>
+
     <br><br><br>
     <h6><font color="white" size="24">Expressing XJTLU's Construct</font></h6>
+
     <h6><font color="#d66">Expressing XJTLU's Construct</font></h6>
 
     <img src="https://static.igem.org/mediawiki/2015/thumb/9/9a/NYU_Shanghai_Chromo_Procedure_2.png/573px-NYU_Shanghai_Chromo_Procedure_2.png">
 
     <img src="https://static.igem.org/mediawiki/2015/thumb/9/9a/NYU_Shanghai_Chromo_Procedure_2.png/573px-NYU_Shanghai_Chromo_Procedure_2.png">
 
     </p>
 
     </p>

Revision as of 00:25, 18 September 2015

Protocols

We built our constructs from pre-made biobrick parts. Our overall conclusion is that 3A assembly is generally inefficient, and an insufficient method for adding small parts (such as a terminator) to a larger construction within pSB1C3. We learned that ratios were extremely important in the process of 3A Assembly, and we made a summary sheet of the equations we used in pre-digest and pre-ligation that accounts for digest dilution and amount needed to ensure results are seen on a gel, not just ligation ratios. We wished we used gibson assembly.


Making Color

Recipes

3A Assembly

Calculations (pdf)