Difference between revisions of "Team:Brasil-USP/Notebook/Calendar"

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−	<br>
−	<br>
−	<br>
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−
−
−
−	<h1>August</h1>
−
−
−	</thead>
−
−	</tbody>
−
−	<table style="width:100%">
−	<tr>
−	<th style="background-color:#bfacc0">Monday</th>
−	<th style="background-color:#bfacc0">Tuesday</th>
−	<th style="background-color:#bfacc0">Wednesday</th>
−	<th style="background-color:#bfacc0">Thursday</th>
−	<th style="background-color:#bfacc0">Friday</th>
−	<th style="background-color:#bfacc0">Saturday</th>
−	<th style="background-color:#bfacc0">Sunday</th>
−	</tr> <tr>
−	<td></td>
−	<td></td>
−	<td></td>
−	<td></td>
−	<td>01/08
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
−	<ul>
−	<li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
−	</ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>02/08</td>
−	<td>03/08</td>
−	</tr>
−	<tr>
−	<td>04/08
−	<ul>
−	<li>Inoculum</li>
−	<ul>
−	<li>E. coli DH5-alpha</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
−	<li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
−	</ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	<ul>
−	<li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
−	</ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
−	<ul>
−	<li>Prepare more inoculum</li>
−	<li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>05//08
−	<ul>
−	<li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
−	<ul>
−	<li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
−	</ul>
−	<li>Inoculum</li>
−	<ul>
−	<li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
−	</ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	<li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li>
−	</ul>
−	</ul>
−	</td>
−	<td>06/08</td>
−	<td>07/08
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
−	<ul>
−	<li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>08/08</td>
−	<td>09/08</td>
−	<td>10/08</td>
−	</tr> <tr>
−	<td>11/08
−	<ul>
−	<li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li>
−	<li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
−	</ul>
−	</td>
−	<td>12/08
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
−	<ul>
−	<li>RBS+qteE</li>
−	<li>RBS+lasR</li>
−	<li>OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard</li>
−	</ul>
−	</ul>
−	</td>
−	<td>13/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>14/08
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li>
−	<ul>
−	<li>RBS+qteE</li>
−	<li>RBS+lasR</li>
−	</ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	<li>OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion</li>
−	</ul>
−	</ul>
−	</td>
−	<td>15/08</td>
−	<td>16/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>17/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	</ul>
−	</ul>
−	</td>
−	</tr> <tr>
−	<td>18/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	</ul>
−	<li>Prepare LB medium (liquid and solid)</li>
−	</ul>
−	</td>
−	<td>19/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>20/08
−	<ul>
−	<li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	</ul>
−	</td>
−	<td>21/08
−	<ul>
−	<li>RBS+qteE, RBS+lasR and Assemblies <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>22/08
−	<ul>
−	<li>Prepare LB medium (liquid and solid)</li>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
−	<ul>
−	<li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li>
−	</ul>
−	</ul>
−	</td>
−	<td>23/08</td>
−	<td>24/08</td>
−	</tr> <tr>
−	<td>25/08
−	<ul>
−	<li>Inoculum</li>
−	<ul>
−	<li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
−	<li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
−	</ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li>
−	<ul>
−	</td>
−	<td>26/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>27/08
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>28/08</td>
−	<td>29/08</td>
−	<td>30/08</td>
−	<td>31/08</td>
−	</tr>
−	</table>
−	<br>
−	<br>
−	<br>
−
−	<h1>September</h1>
−
−
−	</thead>
−
−	<tbody>
−
−	<table style="width:100%">
−	<tr>
−	<th style="background-color:#bfacc0">Monday</th>
−	<th style="background-color:#bfacc0">Tuesday</th>
−	<th style="background-color:#bfacc0">Wednesday</th>
−	<th style="background-color:#bfacc0">Thursday</th>
−	<th style="background-color:#bfacc0">Friday</th>
−	<th style="background-color:#bfacc0">Saturday</th>
−	<th style="background-color:#bfacc0">Sunday</th>
−	</tr> <tr>
−	<td>01/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV.
−	</ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>02/09
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>03/09
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	</ul>
−	</td>
−	<td>04/09
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>05/09</td>
−	<td>06/09</td>
−	<td>07/09</td>
−	</tr>
−	<tr>
−	<td>08/09
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
−	</ul>
−	</td>
−	<td>09/09</td>
−	<td>10/09
−	<ul>
−	<li>The QuickChange protocol didn't work well. So try again with more attention!</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>11/09
−	<ul>
−	<li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>12/09
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li>
−	</ul>
−	<li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li>
−	</ul>
−	</td>
−	<td>13/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	</ul>
−	<li>Prepare LB medium (solid and liquid)</li>
−	</ul>
−	</td>
−	<td>14/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	</ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−	</ul>
−	</td>
−	</tr> <tr>
−	<td>15/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
−	</ul>
−	</td>
−	<td>16/09
−	<ul>
−	<li>Prepare 40% glycerol solution</li>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,
−	<a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a> and
−	<a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>17/09
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−
−	<ul>
−	<li>Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3</li>
−	</ul>
−
−	</td>
−	<td>18/09
−	<ul>
−	<li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li>
−	</ul>
−	<ul>
−	<li>Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry</li>
−	</ul>
−	</td>
−	<td>19/09</td>
−	<td>20/09</td>
−	<td>21/09</td>
−	</tr> <tr>
−	<td>22/09
−	<ul>
−	<li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−
−	</td>
−	<td>23/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
−	</ul>
−	</td>
−	<td>24/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>25/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	</ul>
−	</td>
−	<td>26/09
−	<ul>
−	<li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	<li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>27/09</td>
−	<td>28/09</td>
−	</tr> <tr>
−	<td>29/09
−	<ul>
−	<li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>30/09
−	<ul>
−	<li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	</ul>
−	</td>
−	<td></td>
−	<td></td>
−	<td></td>
−	<td></td>
−	<td></td>
−	</tr>
−	</table>
−	<br>
−	<br>
−	<br>
−
−
−	<h1> October </h1>
−
−	</thead>
−
−	<tbody>
−
−	<table style="width:100%">
−	<tr>
−	<th style="background-color:#bfacc0">Monday</th>
−	<th style="background-color:#bfacc0">Tuesday</th>
−	<th style="background-color:#bfacc0">Wednesday</th>
−	<th style="background-color:#bfacc0">Thursday</th>
−	<th style="background-color:#bfacc0">Friday</th>
−	<th style="background-color:#bfacc0">Saturday</th>
−	<th style="background-color:#bfacc0">Sunday</th>
−	</tr> <tr>
−	<td></td>
−	<td></td>
−	<td>01/10
−	<ul>
−	<li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
−	<ul>
−	<li>Glycerol Stock</li>
−	<li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>02/10</td>
−	<td>03/10</td>
−	<td>04/10</td>
−	<td>05/10</td>
−	</tr>
−	<tr>
−	<td>06/10
−	<ul>
−	<li>Assemnlies <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a>,
−	<a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf">AIV</a>:</li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
−	<a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
−	<ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>07/10
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
−	<a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
−	<li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>08/10
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
−	<a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
−	</ul>
−	<ul>
−	</td>
−	<td>09/10
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
−	<a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
−	<ul>
−	<li>Inoculum</li>
−	</ul>
−	</td>
−	<td>10/10
−	<ul>
−	<li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
−	<a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
−	<a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
−	<ul>
−	<li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
−	</ul>
−	</ul>
−	</td>
−	<td>11/10</td>
−	<td>12/10</td>
−	</tr> <tr>
−	<td>13/10</td>
−	<td>14/10</td>
−	<td>15/10</td>
−	<td>16/10</td>
−	<td>17/10</td>
−	<td>18/10</td>
−	<td>19/10</td>
−	</tr> <tr>
−	<td>20/10</td>
−	<td>21/10</td>
−	<td>22/10</td>
−	<td>23/10</td>
−	<td>24/10</td>
−	<td>25/10</td>
−	<td>26/10</td>
−	</tr> <tr>
−	<td>27/10</td>
−	<td>28/10</td>
−	<td>29/10</td>
−	<td>30/10</td>
−	<td>31/10</td>
−	<td></td>
−	<td></td>
−	</tr>
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Revision as of 02:21, 18 September 2015

Calendar

Notebook

July

© 2015 Brasil-USP Team · iGEM · IFSC · USP

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Monday	Tuesday	Wednesday	Thursday	Friday	Saturday	Sunday
30/06 PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called BBa_K1521001.	01/07 Gel Purification of lasR PCR product	02/07 Transformation of the Biobricks: BBa_K143012 BBa_K081001 BBa_E0840 Inoculum of the Biobricks sent by iGEM HQ: BBa_K316037 BBa_K316018 BBa_K316015	03/07 Transformation of the Biobricks sent by the Imperial College Team: BBa_K316016 BBa_K143031 Miniprep, Quantification of the plasmidial DNA samples and Restriction Analysis: BBa_K316037 BBa_K316018 BBa_K316015	04/07	05/07	06/07 Inoculum: BBa_K143012 BBa_K081001 BBa_E0840
07/07 Miniprep, Quantification and Restriction Analysis: BBa_K143012 BBa_K081001 BBa_E0840 Transformation: BBa_B0015	08/07	09/07 Inoculum: BBa_B0015	10/07 Miniprep, Quantification and Restriction Analysis: BBa_B0015 (restriction analysis fail, repeat) PCR the qteE gene for amplification. We also added the RBS (BBa_K143021,) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000. Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)	11/07 Prepare 40% glycerol solution Transformation: RBS + lasR (BBa_C0079) Gel Purification: RBS + qteE	12/07	13/07
14/07 Assembly:AIII Digestion EXSP Prepare X-gal Prepare more LB medium (solid + liquid)	15/07 Assembly:AIII Quantification of digestion EXSP products Replate RBS+lasR Ligation of PCR qteE in pGEM T-easy vector Transformation: BBa_J04450 Restriction analysis: BBa_B0015	16/07 Transformation: BBa_K823003 RBS+qteE Electrophoresis analysis: BBa_B0015	17/07 Glycerol Stock and Miniprep: BBa_J04450 RBS+lasR BBa_B0015 Digestion: BBa_J04450 (EP) BBa_J04450 (XS) Inoculum: BBa_K823003 Transformation BBa_K143055	18/07 Glycerol Stock and Miniprep: BBa_J04450 BBa_B0015 Gel Purification: BBa_J04450 (EP) BBa_J04450 (XS) Restriction analysis: BBa_K823003	19/07	20/07
21/07 Transformation: BBa_K316016 Inoculum: BBa_K143055 RBS+qteE Assembly KI: Digestion EXSP	22/07 Inoculum: BBa_K316016 Miniprep, Restriction analysis and Digestion: RBS+qteE Assemblies AIII and KI: Gel Purification Ligation	23/07 Assemblies AIII and KI: Transformation Prepare more competent cells Prepare LB medium (solid and liquid) Inoculum BBa_K316016 Prepare RBS+lasR and RBS+qteE for sequencing	24/07 Assemblies AIII and KI: Inoculum Miniprep: BBa_K316016	25/07 Assemblies AIII and KI: Glycerol Stock Miniprep Restriction analysis	26/07	27/07
28/07 Assembly AIII: Restriction analysis (+NdeI enzyme) OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size. Assembly AII, AV and AVI: Digestion EXSP	29/07 >Assembly AII, AV and AVI: Gel Purification Ligation	30/07 >Assembly AII, AV and AVI: Transformation	31/07 Assembly AII, AV and AVI: Inoculum PCR BBa_K143055 for RBS removal OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :) PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector