Difference between revisions of "Team:Brasil-USP/Notebook/Calendar"

Line 5: Line 5:
 
     <div class="jamboreh">
 
     <div class="jamboreh">
  
−
      <h1>June</h1>
+
      <h1>June</h1>
  
  
Line 19: Line 19:
 
     </tr> <tr>
 
     </tr> <tr>
 
     <td valign=top> 01/06
 
     <td valign=top> 01/06
−
                <br>
+
 
−
                 - Agarose gel (RBS_hokD)<br><br>
+
<br>
−
                 - Gel purification (RBS_hokD)<br><br>
+
                 - Agarose gel (RBS_hokD) <br><br>
 +
                 - Gel purification (RBS_hokD) <br><br>
 
                 - Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) <br><br>
 
                 - Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD) <br><br>
 
                 - EXSP Digestion (P0040, Pl_RBS, GFP_R0440) <br><br>
 
                 - EXSP Digestion (P0040, Pl_RBS, GFP_R0440) <br><br>
Line 29: Line 30:
 
                 - Interlab gel purification and ligation <br><br>
 
                 - Interlab gel purification and ligation <br><br>
 
                 <br>
 
                 <br>
 +
 +
 +
 
     </td>
 
     </td>
 
     <td valign=top> 02/06
 
     <td valign=top> 02/06
 
        
 
        
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
<br>      
 +
- Transformation Ptet_I13507<br><br>
 +
- Agarose gel (Pl_RBS, GFP_P0440)<br><br>
 +
- Gel purification (Pl_RBS, GFP_P0440)<br><br>
 +
- Ligation Pl_P0440 (Pl_RBS + GFP_P0440)<br><br>
 +
- Interlab transformation (with wrong antibiotic ampicillin - is possible found in registry the same parts in another backbone BBa_J61002)<br><br>
 +
<br>
 
        
 
        
 
     </td>
 
     </td>
 
     <td valign=top align=left> 03/06
 
     <td valign=top align=left> 03/06
−
     
+
 
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
<br>      
 +
- Transformation (Pr_RBS, 2X_RBS_hokD)<br><br>
 +
- EXSP digestion (P0440, R0040)<br><br>
 +
- Transformation Pl_R0440 (Pl_RBS + GFP_P0440)<br><br>
 +
- Inoculum (Pt_I13507)<br><br>
 +
<br>
 
        
 
        
 
     </td>
 
     </td>
 
     <td valign=top align=left> 04/06
 
     <td valign=top align=left> 04/06
 +
<br>
 +
- Agarose gel (P0440, R0040)<br><br>
 +
- Gel purification (P0440, R0040)<br><br>
 +
- Ligation tet_Ptet (P0440 + R0040)<br><br>
 +
- Inoculum (2X_RBS_hokD)<br><br>
 +
- Miniprep (Pt_I13507)<br><br>
 +
<br>
 
     </td>
 
     </td>
 
     <td valign=top align=left> 05/06
 
     <td valign=top align=left> 05/06
 +
<br>
 +
- Inoculum (R0080, K91403)<br><br>
 +
- EXSP digestion (tet_Term, Pt_E0840)<br><br>
 +
- Miniprep (2X_RBS_hokD)<br><br>
 +
<br>
 +
 +
 
     </td>
 
     </td>
 
     <td valign=top align=left> 06/06
 
     <td valign=top align=left> 06/06
−
    </td>
+
<br>   
 +
- NotI digestion (2X_RBS_hokD, Pr_RBS, Pa_RBS)<br><br>
 +
- Transformation (tet_Ptet)<br><br>
 +
- Agarose gel (2X_RBS_hokD) – positive results<br><br>
 +
- Agarose gel (tet_Term, Pt_E0840)<br><br>
 +
- EXSP digestion (Pt_E0480)<br><br>
 +
- Inoculum (Pl_P0440)<br><br>
 +
- NotI digestion (Pt_I13507) – positive results <br><br>
 +
<br>
 +
 
 +
    </td>
 
     <td valign=top align=left> 07/06
 
     <td valign=top align=left> 07/06
 +
<br>
 +
- Agarose gel (Pr_RBS, Pa_RBS, Pt_E0480) – positive results for Pr_RBS and Pt_E0840)<br><br>
 +
- EXSP digestion (2X_RBS_hokD, B0015)<br><br>
 +
- Ligation Term_E0840 (Pt_E0840 + tet_Term)<br><br>
 +
- Inoculum (tet_Ptet)<br><br>
 +
- Transformation (Term_E0840)<br><br>
 +
- Miniprep (Pl_P0440)<br><br>
 +
- Not digestion (Pl_P0440) – positive results<br><br>
 +
- EXSP digestion (Pl_P0440, Pt_I13507)<br><br>
 +
- Inoculum (GFP_P0440)<br><br>
 +
<br>
 
     </td>
 
     </td>
 
     </tr> <tr>
 
     </tr> <tr>
 
     <td valign=top align=left> 08/06</td>
 
     <td valign=top align=left> 08/06</td>
 +
<br>
 +
- Agarose gel (2X_RBS_hokD, B0015) – B0015 didn’t digest<br><br>
 +
- Gel purification (2X_RBS_hokD)<br><br>
 +
- EXSP digestion (B0015)<br><br>
 +
- Miniprep (tet_Ptet)<br><br>
 +
- NotI digestion (tet_Ptet)<br><br>
 +
- Agarose gel (tet_Ptet, B0015) – positive results<br><br>
 +
- Gel digestion (B0015)<br><br>
 +
- Ligation hokD_Term (2X_RBS_hokD + B0015)<br><br>
 +
- Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br>
 +
- Miniprep (GFP_P0440)<br><br>
 +
- EXSP digestion (GFP_P0440)<br><br>
 +
 +
 +
 +
 +
<br>
 
     <td > 09/06
 
     <td > 09/06
−
     
+
<br>     
−
        <li><font size=2>Transformation (B0034, K1497008, R0010, K914003, R0080, C0040, B0015, R0040, E0840, E0040, P0440, I13507)</font></li>
+
- Agarose gel and gel purification (GFP_P0440)<br><br>
 +
- Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)<br><br>
 +
- Transformation (Pl_I13507)<br><br>
 +
<br>
 
     </td>
 
     </td>
 
     <td valign=top align=left> 10/06
 
     <td valign=top align=left> 10/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Transformation (hokD_Term)<br><br>
−
     
+
- Inoculum (Pl_I13507)<br><br>
 +
- Transformation (GFP_I13507)<br><br>
 +
<br>
 +
 
 
     <td valign=top align=left> 11/06
 
     <td valign=top align=left> 11/06
−
     
+
<br>      
−
          <li><font size=2> iGEM kit arrived!</font></li>
+
- Miniprep (Pl_I13507)<br><br>
−
     
+
<br>  
 
     </td>
 
     </td>
 +
 
     <td valign=top align=left> 12/06
 
     <td valign=top align=left> 12/06
 
        
 
        
−
        <li><font size=2> iGEM kit arrived!</font></li>
 
 
        
 
        
 
     <td valign=top align=left> 13/06
 
     <td valign=top align=left> 13/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Inoculum (GFP_I13507)<br><br>
−
     
+
- NotI digestion (Pl_I13507)<br><br>
 +
- Agarose gel (Pl_I13507) – negative results<br><br>
 +
<br>
 
     </td>
 
     </td>
−
    <td valign=top align=left> 14/06
+
   
−
     
+
<td valign=top align=left> 14/06
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
 
 +
<br>      
 +
- Miniprep (GFP_I13507)<br><br>
 +
- NotI digestion (GFP_I13507) – negative results<br><br>
 +
- EXSP digestion (Pl_P0440, Pt_I13507)<br><br>
 +
- Inoculum (GFP_P0440, Pt_I13507)<br><br>
 +
<br>
 
      
 
      
 
     <tr>
 
     <tr>
 
     <td valign=top align=left> 15/06
 
     <td valign=top align=left> 15/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Agarose gel (Pl_P0440, Pt_I13507)<br><br>
 +
- Gel purification (Pl_P0440, Pt_I13507)<br><br>
 +
- Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)<br><br>
 +
- Miniprep (GFP_P0440, Pt_I13507)<br><br>
 +
- EXSP digestion (GFP_P0440, Pt_I13507)<br><br>
 +
<br>
 +
 
 
        
 
        
 
     </td>
 
     </td>
 
     <td valign=top align=left> 16/06
 
     <td valign=top align=left> 16/06
 
        
 
        
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
<br>
 +
- Agarose gel and gel purification (GFP_P0440, Pt_I13507)<br><br>
 +
- Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)<br><br>
 +
- Transformation (Pl_I13507)<br><br>
 +
<br>
 +
 
 
        
 
        
 
     <td valign=top align=left> 17/06
 
     <td valign=top align=left> 17/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Transformation (GFP_I13507)<br><br>
−
     
+
- Inoculum (Pt_Term, Pl_I1350)<br><br>
 +
<br>     
 +
 
 +
 
 
     </td>
 
     </td>
 
     <td valign=top align=left> 18/06
 
     <td valign=top align=left> 18/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Miniprep (Pt_Term, Pl_I13507)<br><br>
−
     
+
- NotI digestion (Pt_Term, Pl_I13507)<br><br>
 +
- Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term<br><br>
 +
<br>     
 
     <td valign=top align=left> 19/06
 
     <td valign=top align=left> 19/06
−
     
+
 
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
 
        
 
        
 
     </td>
 
     </td>
Line 105: Line 201:
 
        
 
        
 
     <td valign=top align=left> 21/06
 
     <td valign=top align=left> 21/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Inoculum (GFP_I13507, Pl_I13507)<br><br>
−
     
+
- New interlab transformations<br><br>
 +
<br>
 
     </td>
 
     </td>
 
     </tr>     
 
     </tr>     
 
     <td valign=top align=left> 22/06
 
     <td valign=top align=left> 22/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Miniprep (GFP_I13507, Pl_I13507)<br><br>
−
     
+
- NotI digestion (GFP_I13507, Pl_I13507)<br><br>
 +
- EXSP digestion (Pr_RBS, R0080)<br><br>
 +
- Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS)<br><br>
 +
- Gel purification (Pr_RBS)<br><br>
 +
- Inoculum (GFP_I13507, Pl_I13507)<br><br>
 +
- Interlab inocula<br><br>
 +
<br>
 
     <td valign=top align=left> 23/06
 
     <td valign=top align=left> 23/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Agarose gel (R0080)<br><br>
 +
- Miniprep (GFP_I13507, Pl_I13507)<br><br>
 +
- NotI digestion (GFP_I13507, Pl_I13507)<br><br>
 +
- Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507<br><br>
 +
- EXSP digestion (GFP_I13507)<br><br>
 +
- Interlab miniprep and restriction analysis<br><br>
 +
<br>
 +
 
 
        
 
        
 
     </td>
 
     </td>
 
     <td valign=top align=left> 24/06
 
     <td valign=top align=left> 24/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Miniprep (B0015, Pt_P0440)<br><br>
 +
- EXSP digestion (2X_RBS_hokD, B0015)<br><br>
 +
- Transformation (Pa_RBS)<br><br>
 +
- Agarose gel of restriction analysis: devices confirmed<br><br>
 +
<br>
 +
 
 
        
 
        
 
     <td valign=top align=left> 25/06
 
     <td valign=top align=left> 25/06
−
     
+
 
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
<br>      
 +
- agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest<br><br>
 +
<br>
 
        
 
        
 
     </td>
 
     </td>
 
     <td valign=top align=left> 26/06
 
     <td valign=top align=left> 26/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Agarose gel and gel purification (GFP_I13507)<br><br>
−
     
+
- Ligation Pr_I13507 (Pr_RBS + GFP_I13507)<br><br>
 +
<br>
 
     <td valign=top align=left> 27/06
 
     <td valign=top align=left> 27/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- EXSP digestion (Pl_RBS, Pt_Term)<br><br>
 +
- Transformation (Pr_I13507, Pa_RBS)<br><br>
 +
<br>
 
        
 
        
 
     </td>
 
     </td>
 
     <td valign=top align=left> 28/06
 
     <td valign=top align=left> 28/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- Agarose gel (Pl_RBS, Pt_Term)<br><br>
−
     
+
ligation Pr_I13507(Pr_RBS + Pt_Term)<br><br>
 +
<br>
 
     </td>
 
     </td>
 
     <tr>
 
     <tr>
Line 146: Line 267:
 
     <td valign=top align=left> 29/06
 
     <td valign=top align=left> 29/06
 
        
 
        
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
<br>      
−
     
+
- EXSP digestion (B0015, 2X_RBS_hokD)<br><br>
 +
- agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest<br><br>
 +
- EXSP digestion overnight (2X_RBS_hokD)<br><br>
 +
- transformation (Pr_Term, Pl_RBS)<br><br>
 +
<br>
 
     </td>
 
     </td>
 
     <td valign=top align=left> 30/06
 
     <td valign=top align=left> 30/06
−
     
+
<br>     
−
        <li><font size=2> iGEM kit arrived!</font></li>
+
- inoculum (Pl_RBS, Pa_RBS)<br><br>
 +
- agarose gel (B0015, 2X_RBS_hokD) - Digestion worked!<br><br>
 +
- ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD)<br><br>
 +
<br>
 +
 
 
        
 
        
 
     </td>
 
     </td>
Line 161: Line 290:
 
      
 
      
 
   </table>
 
   </table>
 +
 
    
 
    
  

Revision as of 03:58, 18 September 2015

Notebook

Calendar

June


- Agarose gel (2X_RBS_hokD, B0015) – B0015 didn’t digest

- Gel purification (2X_RBS_hokD)

- EXSP digestion (B0015)

- Miniprep (tet_Ptet)

- NotI digestion (tet_Ptet)

- Agarose gel (tet_Ptet, B0015) – positive results

- Gel digestion (B0015)

- Ligation hokD_Term (2X_RBS_hokD + B0015)

- Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)

- Miniprep (GFP_P0440)

- EXSP digestion (GFP_P0440)


Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/06
- Agarose gel (RBS_hokD)

- Gel purification (RBS_hokD)

- Ligation 2X_RBS_hokD (RBS_hokD + RBS_hokD)

- EXSP Digestion (P0040, Pl_RBS, GFP_R0440)

- Agarose gel (P0040)

- Gel purification (P0040)

- Ligation Pt_I13507(P0040 + I13507)

- Interlab gel purification and ligation


02/06
- Transformation Ptet_I13507

- Agarose gel (Pl_RBS, GFP_P0440)

- Gel purification (Pl_RBS, GFP_P0440)

- Ligation Pl_P0440 (Pl_RBS + GFP_P0440)

- Interlab transformation (with wrong antibiotic ampicillin - is possible found in registry the same parts in another backbone BBa_J61002)


 03/06
- Transformation (Pr_RBS, 2X_RBS_hokD)

- EXSP digestion (P0440, R0040)

- Transformation Pl_R0440 (Pl_RBS + GFP_P0440)

- Inoculum (Pt_I13507)


 04/06
- Agarose gel (P0440, R0040)

- Gel purification (P0440, R0040)

- Ligation tet_Ptet (P0440 + R0040)

- Inoculum (2X_RBS_hokD)

- Miniprep (Pt_I13507)


 05/06
- Inoculum (R0080, K91403)

- EXSP digestion (tet_Term, Pt_E0840)

- Miniprep (2X_RBS_hokD)


 06/06
- NotI digestion (2X_RBS_hokD, Pr_RBS, Pa_RBS)

- Transformation (tet_Ptet)

- Agarose gel (2X_RBS_hokD) – positive results

- Agarose gel (tet_Term, Pt_E0840)

- EXSP digestion (Pt_E0480)

- Inoculum (Pl_P0440)

- NotI digestion (Pt_I13507) – positive results


07/06
- Agarose gel (Pr_RBS, Pa_RBS, Pt_E0480) – positive results for Pr_RBS and Pt_E0840)

- EXSP digestion (2X_RBS_hokD, B0015)

- Ligation Term_E0840 (Pt_E0840 + tet_Term)

- Inoculum (tet_Ptet)

- Transformation (Term_E0840)

- Miniprep (Pl_P0440)

- Not digestion (Pl_P0440) – positive results

- EXSP digestion (Pl_P0440, Pt_I13507)

- Inoculum (GFP_P0440)
08/06 09/06
- Agarose gel and gel purification (GFP_P0440)

- Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)

- Transformation (Pl_I13507)


 10/06
- Transformation (hokD_Term)

- Inoculum (Pl_I13507)

- Transformation (GFP_I13507)


 11/06
- Miniprep (Pl_I13507)


 12/06 13/06
- Inoculum (GFP_I13507)

- NotI digestion (Pl_I13507)

- Agarose gel (Pl_I13507) – negative results


 14/06
- Miniprep (GFP_I13507)

- NotI digestion (GFP_I13507) – negative results

- EXSP digestion (Pl_P0440, Pt_I13507)

- Inoculum (GFP_P0440, Pt_I13507)


15/06
- Agarose gel (Pl_P0440, Pt_I13507)

- Gel purification (Pl_P0440, Pt_I13507)

- Ligation Pl_I13507 (Pl_P0440 + Pt_I13507)

- Miniprep (GFP_P0440, Pt_I13507)

- EXSP digestion (GFP_P0440, Pt_I13507)


 16/06
- Agarose gel and gel purification (GFP_P0440, Pt_I13507)

- Ligation GFP_I13507 (GFP_P0440 + Pt_I13507)

- Transformation (Pl_I13507)


 17/06
- Transformation (GFP_I13507)

- Inoculum (Pt_Term, Pl_I1350)


 18/06
- Miniprep (Pt_Term, Pl_I13507)

- NotI digestion (Pt_Term, Pl_I13507)

- Agarose gel (Pt_Term, Pl_I13507) – positive results for Pt_Term


 19/06 20/06
  • iGEM kit arrived!
    		•  21/06
    - Inoculum (GFP_I13507, Pl_I13507)

    - New interlab transformations
    22/06
    - Miniprep (GFP_I13507, Pl_I13507)

    - NotI digestion (GFP_I13507, Pl_I13507)

    - EXSP digestion (Pr_RBS, R0080)

    - Agarose gel (GFP_I13507, Pl_I13507, Pr_RBS)

    - Gel purification (Pr_RBS)

    - Inoculum (GFP_I13507, Pl_I13507)

    - Interlab inocula


    		 23/06
    - Agarose gel (R0080)

    - Miniprep (GFP_I13507, Pl_I13507)

    - NotI digestion (GFP_I13507, Pl_I13507)

    - Agarose gel (GFP_I13507, Pl_I13507) – Positive results for GFP_I13507

    - EXSP digestion (GFP_I13507)

    - Interlab miniprep and restriction analysis


    		 24/06
    - Miniprep (B0015, Pt_P0440)

    - EXSP digestion (2X_RBS_hokD, B0015)

    - Transformation (Pa_RBS)

    - Agarose gel of restriction analysis: devices confirmed


    		 25/06
    - agarose gel (2X_RBS_hokD, B0015) - B0015 did not digest


    		 26/06
    - Agarose gel and gel purification (GFP_I13507)

    - Ligation Pr_I13507 (Pr_RBS + GFP_I13507)


    		 27/06
    - EXSP digestion (Pl_RBS, Pt_Term)

    - Transformation (Pr_I13507, Pa_RBS)


    		 28/06
    - Agarose gel (Pl_RBS, Pt_Term)

    ligation Pr_I13507(Pr_RBS + Pt_Term)
    29/06
    - EXSP digestion (B0015, 2X_RBS_hokD)

    - agarose gel (B0015, 2X_RBS_hokD) - 2X_RBS_hokD didn’t digest

    - EXSP digestion overnight (2X_RBS_hokD)

    - transformation (Pr_Term, Pl_RBS)


    		 30/06
    - inoculum (Pl_RBS, Pa_RBS)

    - agarose gel (B0015, 2X_RBS_hokD) - Digestion worked!

    - ligation 2X_hokD_Term (B0015 + 2X_RBS_hokD) P0440_hokD(P0440_term + 2X_RBS_hokD)


    		 31/06
  • iGEM kit arrived!
    		•

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