Difference between revisions of "Team:UMaryland/Results"

Revision as of 05:13, 18 September 2015

Considerations

PCR of Hok-Sok Construct

Amplification of the Hok-Sok cassette is difficult due to the high inherent secondary structure in the construct. Hok ssDNA is capable of naturally folding into a stable secondary structure, resulting in early terminations and other side products. We recommend that a reaction buffer suitable for difficult templates be used, such as Phusion GC buffer + added DMSO.

Measuring OD of RFP Cultures

Due to the close proximity of the emission wavelength of RFP (584 nm) and the classical absorbance wavelength for measuring cell density (600 nm), it is difficult to accurately determine the cell density of cultures that are expressing RFP. Given more time to calibrate our testing measurements, we would either have used an alternative wavelength for measuring OD (>600 nm), used a hemocytometer as an alternate counting method, or switched to GFP as an alternative fluorescent marker whose emission wavelength differs from 600 nm by a greater amount.

Project Results

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Clearly and objectively describe the results of your work.

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Considerations for replicating the experiments

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A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

Inspiration

See how other teams presented their results.

@@ Line 112: / Line 112: @@
 <h1 align="center"><b>Analysis of Fluorescence Loss</b></h1>
-<img src = "https://static.igem.org/mediawiki/2015/thumb/f/f2/UMaryland_Group_B_090615.JPG/800px-UMaryland_Group_B_090615.JPG">
+<p><img src = "https://static.igem.org/mediawiki/2015/thumb/f/f2/UMaryland_Group_B_090615.JPG/800px-UMaryland_Group_B_090615.JPG"></p>
 <p style = "font-size:18px">Over time, colonies appear to stop producing RFP.</p>
@@ Line 118: / Line 118: @@
 <br>
 <br>
-<img src = "https://static.igem.org/mediawiki/2015/2/26/UMaryland_Group_A_090615.JPG" width = "100% height = "100%"/>
+<p><img src = "https://static.igem.org/mediawiki/2015/2/26/UMaryland_Group_A_090615.JPG" width = "100% height = "100%"/></p>
 <p style = "font-size:18px"> This effect occurs even when the culture is under chloramphenicol pressure.</p>
 <br>
 <br>
 <br>
-<img src = "https://static.igem.org/mediawiki/2015/thumb/b/bd/UMaryland_Group_D_090615.JPG/800px-UMaryland_Group_D_090615.JPG">
+<p><img src = "https://static.igem.org/mediawiki/2015/thumb/b/bd/UMaryland_Group_D_090615.JPG/800px-UMaryland_Group_D_090615.JPG"></p>
 <p style = "font-size:18px"> This effect occurs much more slowly when the Hok-Sok cassette is adjacent to the RFP construct.</p>

Difference between revisions of "Team:UMaryland/Results"

Revision as of 05:13, 18 September 2015

Plating Tests

Negative Control (No Pressure)

Chloramphenicol Pressure Only

Chloramphenicol + Hok-Sok Pressure

Hok-Sok Pressure Only

Growth Curve

Fluorescence Studies

Analysis of Fluorescence Loss

Future Plans

Considerations

Project Results

What should this page contain?

Project Achievements

Inspiration