Difference between revisions of "Team:UMaryland/Notebook"

Line 427: Line 427:
 
Hok/Sok  
 
Hok/Sok  
 
<ul class="a">
 
<ul class="a">
   <li>- sequence confirmed through sequencing </li>
+
   <li>- const_promoter + RBS + RFP were replated from last week but produced no colonies </li>
   <li>- failed to extract using French press, FPLC and SDS-Page </li>
+
   <li>- re-transformed original and new 3A assembly which produced the correct sequence  </li>
   <li>- could be due to high arabinose induction (OD of 1) </li>
+
  <li>- ligated const_promoter:QD-RFP in PSB1C3 </li>
 +
  <li>- performed a 3A assembly of Hok/Sok plasmid with QD-RFP downstream in PSB1K3 </li>
 +
   <li>- site directed mutagenesis of quick degrading GFP (Bba_K750000) failed </li>
 +
<ul class="a">
 +
  <li>- there was no change from the original sequence </li></li>
 
</ul>
 
</ul>
const_promoter + RBS + RFP were replated from last week but produced no colonies
+
 
<br>
+
 
re-transformed original and new 3A assembly which produced the correct sequence
+
<br>
+
ligated const_promoter:QD-RFP in PSB1C3
+
<br>
+
performed a 3A assembly of Hok/Sok plasmid with QD-RFP downstream in PSB1K3
+
<br>
+
site directed mutagenesis of quick degrading GFP (Bba_K750000)  failed
+
<br>
+
there was no change from the original sequence
+
 
<br>
 
<br>
 
<p style="font-size:24px;text-align:left;text-decoration: underline;">
 
<p style="font-size:24px;text-align:left;text-decoration: underline;">

Revision as of 09:32, 18 September 2015