Difference between revisions of "Team:BABS UNSW Australia/Parts"
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<il>BBa_K1677103: LysM-INV (Lactococcus)</il> | <il>BBa_K1677103: LysM-INV (Lactococcus)</il> | ||
| − | <ul><li>The signal peptide region of <i>Listeria monocytogenes</i> was exchanged for a lactococcal signal peptide. The aim of this is localise the invasin protein to the outer membrane when used in <i>Lactococcus</i> species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in <i>L. lactis</i>. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions. | + | <ul><li>This protein-coding part encodes invasin tailored for use in <i>Lactococcus</i> species.The signal peptide region of <i>Listeria monocytogenes</i> was exchanged for a lactococcal signal peptide. The aim of this is localise the invasin protein to the outer membrane when used in <i>Lactococcus</i> species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in <i>L. lactis</i>. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.</li> |
</ul> | </ul> | ||
<il>BBa_K1677101: LLO (Lactococcus)</il> | <il>BBa_K1677101: LLO (Lactococcus)</il> | ||
| − | <ul> | + | <ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells. </li> |
</ul> | </ul> | ||
<img src="https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png" style="max-width:40%;"> | <img src="https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png" style="max-width:40%;"> | ||
Revision as of 15:21, 18 September 2015
Part Documentation
Lactoccocus Parts
- This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a lactococcal signal peptide. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.
- This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells.
Parts Table
<groupparts>iGEM015 BABS_UNSW_Australia</groupparts>