Difference between revisions of "Team:BABS UNSW Australia/Parts"

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<h4>Lactoccocus Parts </h4>
 
<h4>Lactoccocus Parts </h4>
 
<ul>
 
<ul>
−
<il>BBa_K1677103: LysM-INV (Lactococcus)</il>
+
<il>BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)</il>
−
<ul><li>This protein-coding part encodes invasin tailored for use in <i>Lactococcus</i> species.The signal peptide region of <i>Listeria monocytogenes</i> was exchanged for a lactococcal signal peptide. The aim of this is localise the invasin protein to the outer membrane when used in <i>Lactococcus</i> species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in <i>L. lactis</i>. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.</li>
+
<ul><li>This protein-coding part encodes invasin tailored for use in <i>Lactococcus</i> species.The signal peptide region of <i>Listeria monocytogenes</i> was exchanged for a signal peptide <i>Lactobacillus plantarum</i>. The aim of this is localise the invasin protein to the outer membrane when used in <i>Lactococcus</i> species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in <i>L. lactis</i>. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.</li>
 
</ul>
 
</ul>
−
<il>BBa_K1677101: LLO (Lactococcus)</il>
+
<il>BBa_K1677101: Listeriolysin (Lactococcus)</il>
−
<ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells. </li>
+
<ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap. </li>
 
</ul>
 
</ul>
−
<img src="https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png" style="max-width:40%;">
+
<img src="https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png" style="max-width:30%;">
 
</p>
 
</p>
 +
 +
<h4>Pseudoknots</h4>
 +
<ul>BBa_K1677391</ul>
 +
<ul>BBa_K1677369</ul>
 +
<ul>BBa_K1677743</ul>
 +
 +
<ul>BBa_K1677800: IPTG inducible promoter with IBV pseudoknot</ul>
 +
<ul>BBa_K1677801: IPTG inducible promoter with TGV pseudoknot</ul>
 +
<ul>BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot</ul>
 +
<il>The above three parts
  
 
<h4>Parts Table </h4>
 
<h4>Parts Table </h4>

Revision as of 15:34, 18 September 2015

Part Documentation

Lactoccocus Parts

    BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)
    • This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a signal peptide Lactobacillus plantarum. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.
    BBa_K1677101: Listeriolysin (Lactococcus)
    • This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap.

    Pseudoknots

      BBa_K1677391
      BBa_K1677369
      BBa_K1677743
      BBa_K1677800: IPTG inducible promoter with IBV pseudoknot
      BBa_K1677801: IPTG inducible promoter with TGV pseudoknot
      BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot
    The above three parts

    Parts Table

    <groupparts>iGEM015 BABS_UNSW_Australia</groupparts>