Difference between revisions of "Team:BABS UNSW Australia/Parts"
MackenzieLR (Talk | contribs) |
MackenzieLR (Talk | contribs) |
||
Line 38: | Line 38: | ||
<h4>Lactoccocus Parts </h4> | <h4>Lactoccocus Parts </h4> | ||
<ul> | <ul> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png" style="max-width:30%;"> | ||
<il>BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)</il> | <il>BBa_K1677103: Invasin with LysM signal peptide (Lactococcus)</il> | ||
Line 45: | Line 46: | ||
<ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap. </li> | <ul><li>This part is listeriolysin codon-optimised for expression in <i>Lactococcus</i>. By co-expressing with the above LysM-INV, <i>Lactococcus</i> species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap. </li> | ||
</ul> | </ul> | ||
− | + | ||
</p> | </p> | ||
Line 52: | Line 53: | ||
<il>BBa_K1677391: IBV Pseudoknot</il> | <il>BBa_K1677391: IBV Pseudoknot</il> | ||
+ | <ul><li>Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.</ul></li> | ||
<il>BBa_K1677369: MMTV Pseudoknot</il> | <il>BBa_K1677369: MMTV Pseudoknot</il> | ||
+ | <ul><li>Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.</ul></li> | ||
<il>BBa_K1677743: TGV Pseudoknot</il> | <il>BBa_K1677743: TGV Pseudoknot</il> | ||
+ | <ul><li>Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.</ul></li> | ||
<ul> The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.</ul> | <ul> The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.</ul> | ||
Revision as of 16:17, 18 September 2015
![](https://static.igem.org/mediawiki/2015/5/58/BABS_UNSW_Australia_parts.png)
Part Documentation
Lactoccocus Parts
- This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a signal peptide Lactobacillus plantarum. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis. This part was assembled through Gibson assembly of three g-blocks designed with 20-30bp overlapping regions.
- This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells. This part was assembled through overlap PCR of two g-blocks with a 20bp overlap.
- Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.
- Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.
- Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.
- The above three parts
![](https://static.igem.org/mediawiki/2015/9/99/BABS_UNSW_Australia_LactoGblockDeusExMachina.png)
Pseudoknots
- The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.
Parts Table
<groupparts>iGEM015 BABS_UNSW_Australia</groupparts>