Difference between revisions of "Team:UMaryland/Notebook"

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<b>Week 1</b></a>
 
<b>Week 1</b></a>
 
<p style="font-size:24px;text-align:left;text-decoration: underline;">
 
<p style="font-size:24px;text-align:left;text-decoration: underline;">
−
Miraculin:
+
First week review
−
<br>
+
<ul>
−
<ul class="a">
+
<li>Cleaned the lab</li>
−
  <li>- ligated the pBAD promoter in PSB1C3 with the miraculin gene in PSB1C3 using 3A assembly</li>
+
<li>moved in iGEM materials</li>
−
  <li>- plate had colonies</li>
+
<li>Miniprep<li>
 +
<li>analyzed sequences</li>
 +
<li>prepared 3A assembly clone of pBAD-miraculin</li>
 
</ul>
 
</ul>
 +
<ul>
 +
<li>3A assembly</li>
 +
<li>two backbones</li>
 +
<li>pSB1C3</li>
 +
<li>pBAD promoter + genes for miraculin</li>
 +
<li>cut out pBAD with 2 enzymes (EcoRI and SpeI) and cut out miraculin (XbaI and PstI)</li>
 +
<li>want to put pBAD in front of miraculin</li>
 +
<li>put into backbone w/ different antibiotic resistance than original backbone: pSB1A3 (means of selection)</li>
 +
<li>plate had colonies<li>
 +
 +
<li>Human Practices<li>
 +
Kevin got in contact w/ Bioacademy program @ Northwest High School
 +
Trying to set up a talk for this Wednesday
 +
Kevin + Adam + Dylan + Juhye going
 +
goal to target various age groups
 +
create game
 +
fundamentals of genetics via presentations, arts, etc.
 +
Conference scheduled for June 25th
 +
4 teams confirmed: UVA, UMBC, Stonybrook, Duke
 +
contact iGEM headquarters to inform them about meetup → Robert
 +
<p class=MsoNormal>Kevin got in contact w/ <span class=SpellE>Bioacademy</span>
 +
program @ Northwest High School <o:p></o:p></p>
 +
 +
<p class=MsoNormal>Trying to set up a talk for this <span class=SpellE><span
 +
class=GramE>wednesday</span></span> <o:p></o:p></p>
 +
 +
<p class=MsoNormal>Kevin + Adam + Dylan + <span class=SpellE>Juhye</span> going
 +
<o:p></o:p></p>
 +
 +
<p class=MsoNormal><span class=GramE>goal</span> to target various age groups <o:p></o:p></p>
 +
 +
<p class=MsoNormal><span class=GramE>create</span> game <o:p></o:p></p>
 +
 +
<p class=MsoNormal><span class=GramE>fundamentals</span> of genetics via
 +
presentations, arts, etc. <o:p></o:p></p>
 +
 +
<p class=MsoNormal>Conference scheduled for June 25th <o:p></o:p></p>
 +
 +
<p class=MsoNormal>4 teams confirmed: UVA, UMBC, <span class=SpellE>Stonybrook</span>,
 +
<span class=GramE>Duke</span> <o:p></o:p></p>
 +
 +
<p class=MsoNormal><span class=GramE>contact</span> <span class=SpellE>iGEM</span>
 +
headquarters to inform them about meetup &#8594; Robert</p>
 +
  
 
<br>
 
<br>

Revision as of 18:20, 18 September 2015