Difference between revisions of "Team:ETH Zurich/Achievements"

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  <td><p>We Improved and characterized variants of the <i>E. coli</i> <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Characterization_of_the_LldR_promoter">lldPRD-operon promoter</a> based on the natural version (<a href="http://parts.igem.org/Part:BBa_K822000">BBa_K822000</a>)on which there is only a limited amount of information available in the Parts Registry and in the literature. The characterization of a <a href="2015.igem.org/wiki/index.php?title=Team:ETH_Zurich/Results#inMenu"> synthetic promoter library</a> yielded promoter variants that far outperform the wild type LldPRD promoter.</p></td>
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  <td><p>We Improved and characterized variants of the <i>E. coli</i> <a href="https://2015.igem.org/Team:ETH_Zurich/Results#Characterization_of_the_LldR_promoter">lldPRD-operon promoter</a> based on the natural version (<a href="http://parts.igem.org/Part:BBa_K822000">BBa_K822000</a>), on which there is only a limited amount of information available in the Parts Registry and in the literature. The characterization of a <a href="2015.igem.org/wiki/index.php?title=Team:ETH_Zurich/Results#inMenu"> synthetic promoter library</a> yielded promoter variants that far outperform the wild type LldPRD promoter.</p></td>
 
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Revision as of 19:19, 18 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

Achievements

We are proud to announce that we accomplished the following objectives:

General Achievements

Medal Criteria

We registered for iGEM, had a great summer so far, and now we are looking forward to attending the Giant Jamboree!

Going for it!

We completed and submitted the Judging Form.

We created a description of our project in time.

We documented all the parts taken from the Registry of Standard Biological Parts, of which two were redesigned and newly characterized.

We are going to present a poster and give a talk at the Giant Jamboree.

Going for it!

We created this website for you to learn about every aspect of our iGEM project.

We documented and submitted two new basic parts to the iGEM parts registry and created a part collection with 14 parts.

These new parts we also submitted to the iGEM Parts Registry.

We characterized two newly designed hybrid promoters and were able to show that one of our combined promoters, Plac-lldR (K1847010), reacts in a clear AND gate fashion to a combination of lactate and IPTG. To our knowledge, combining these two elements has never been attempted before.

Our Human Practices Efforts:

We visited two different elementary schools, thaught the children about what DNA is, performed experiments with them and published an article about it in the local newspaper.

We informed the ETH-student magazine polykum about iGEM and gave an interview.

We contributed to the Newsletters from Amoys team, met with the Darmstadt team, conducted a survey together with the EPFL and provided Colombias team with protocols and troubleshooting advice for their transformations.

More Human Practices Efforts:

We interviewed many different experts from various fields: medical doctors, an expert from the ethics commission of the ETH Zurich, the founder of a startup biotech company as well as an expert in patent law and integrated their advice and ideas into our project design.

We collaborated with the team from Stockholm by testing their constructs.

We Improved and characterized variants of the E. coli lldPRD-operon promoter based on the natural version (BBa_K822000), on which there is only a limited amount of information available in the Parts Registry and in the literature. The characterization of a synthetic promoter library yielded promoter variants that far outperform the wild type LldPRD promoter.

Further Experimental Achievements

  • We designed a chip for future application of our MicroBeacon E. coli.

  • We participated in the interlab study.

  • Our experiments complied with the safety instructions at the Department of Biosystems Science and Engineering D-BSSE in Basel where our lab is situated.

Modeling Achievements

We would like to thank our sponsors