Difference between revisions of "Team:Freiburg/Materials"

(Created page with "{{Freiburg/CSS}} {{Freiburg/Menubar}} {{Freiburg/wiki_content_start}} <html> <!-- content goes in here --> <div class="content_box"> <h2> Instruments </h2> <table class="ta...")
 
Line 6: Line 6:
 
<!-- content goes in here -->
 
<!-- content goes in here -->
 
<div class="content_box">
 
<div class="content_box">
 +
 +
<h1> Materials </h1>
 +
<br>
  
 
<h2> Instruments </h2>
 
<h2> Instruments </h2>
 +
<br>
  
 
<table class="tableizer-table">
 
<table class="tableizer-table">
−
<tr class="tableizer-firstrow"><th>Thermocycler</th><th>Thriller </th><th>Peqlab</th><th>Erlangen</th><th>Germany</th></tr>
+
<tr class="tableizer-firstrow"><th>Device</th><th>Name </th><th>Manufacturer</th><th></th><th></th></tr>
 +
<tr><td>Thermocycler</td><td>Thriller</td><td>Peqlab</td><td>Erlangen</td><td>Germany</td></tr
 
  <tr><td>&nbsp;</td><td>91-D 1200</td><td>Peqlab</td><td>Erlangen</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>91-D 1200</td><td>Peqlab</td><td>Erlangen</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Thermomixer comfort</td><td>Eppendorf</td><td>Hamburg</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Thermomixer comfort</td><td>Eppendorf</td><td>Hamburg</td><td>Germany</td></tr>
Line 17: Line 22:
 
  <tr><td>&nbsp;</td><td>Centrifuge 5804 R</td><td>Eppendorf</td><td>Hamburg</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Centrifuge 5804 R</td><td>Eppendorf</td><td>Hamburg</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Centrifuge Avanti J-26 XP</td><td>BeckmanCoulter</td><td>Brea</td><td>USA</td></tr>
 
  <tr><td>&nbsp;</td><td>Centrifuge Avanti J-26 XP</td><td>BeckmanCoulter</td><td>Brea</td><td>USA</td></tr>
−
  <tr><td>Heizplatten/Schüttler etc</td><td>MR Hei Standard</td><td>Heidolph</td><td>Schwabach</td><td>Germany</td></tr>
+
  <tr><td>Heater/Mixer etc</td><td>MR Hei Standard</td><td>Heidolph</td><td>Schwabach</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Duomax 1030</td><td>Heidolph</td><td>Schwabach</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Duomax 1030</td><td>Heidolph</td><td>Schwabach</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Variable-speed Rock’n roller Model L-202</td><td>Labinco BV</td><td>DG Breda</td><td>Niederlande</td></tr>
 
  <tr><td>&nbsp;</td><td>Variable-speed Rock’n roller Model L-202</td><td>Labinco BV</td><td>DG Breda</td><td>Niederlande</td></tr>
 
  <tr><td>&nbsp;</td><td>Rotamax 120</td><td>Heidolph</td><td>Schwabach</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Rotamax 120</td><td>Heidolph</td><td>Schwabach</td><td>Germany</td></tr>
−
<tr><td>Incubator</td><td>?</td><td>Memmert</td><td>Schwabach</td><td>Germany</td></tr>
 
 
  <tr><td>&nbsp;</td><td>Innova 44</td><td>Eppendorf</td><td>Hamburg</td><td>Germany</td></tr>
 
  <tr><td>&nbsp;</td><td>Innova 44</td><td>Eppendorf</td><td>Hamburg</td><td>Germany</td></tr>
 
  <tr><td>Vortex</td><td> Genius 3</td><td>IKA</td><td>Staufen</td><td>Germany</td></tr>
 
  <tr><td>Vortex</td><td> Genius 3</td><td>IKA</td><td>Staufen</td><td>Germany</td></tr>
Line 41: Line 45:
 
</table>
 
</table>
  
−
<h2> Chemicals </h2>
 
  
 +
<br>
 +
<br>
 +
<h2> Chemicals </h2>
 +
<br>
 
<table class="tableizer-table">
 
<table class="tableizer-table">
−
<tr class="tableizer-firstrow"><th>Phosphoenolpyruvic acid (monopotassium salt)</th><th>Roche</th><th>Basel</th><th>Schweiz</th></tr>
+
<tr class="tableizer-firstrow"><th>Name</th><th>Manufacturer</th><th></th><th></th></tr>
 +
<tr><td>Phosphoenolpyruvic acid (monopotassium salt)</td><td>Roche</td><td>Basel</td><td>Switzerland</td></tr>
 
  <tr><td>Sodium creatine phosphate dibasic tetrahydrate</td><td>Sigma</td><td>St. Louis</td><td>USA</td></tr>
 
  <tr><td>Sodium creatine phosphate dibasic tetrahydrate</td><td>Sigma</td><td>St. Louis</td><td>USA</td></tr>
 
  <tr><td>Magnesium acetate tetrahydrate (Mg(Oac)2)</td><td>Sigma</td><td>St. Louis</td><td>USA</td></tr>
 
  <tr><td>Magnesium acetate tetrahydrate (Mg(Oac)2)</td><td>Sigma</td><td>St. Louis</td><td>USA</td></tr>
Line 79: Line 87:
 
  <tr><td>Phosphate buffered saline</td><td>Sigma</td><td>St. Louis</td><td>USA</td></tr>
 
  <tr><td>Phosphate buffered saline</td><td>Sigma</td><td>St. Louis</td><td>USA</td></tr>
 
</table>
 
</table>
 +
 +
<br>
 +
<br>
  
 
<h2> Media and Buffers </h2>
 
<h2> Media and Buffers </h2>
 +
<br>
  
 
<table class="tableizer-table">
 
<table class="tableizer-table">
−
<tr class="tableizer-firstrow"><th>Phusion Buffer</th><th>NEB (New England Biolabs)</th><th>Ipswitch</th><th>USA</th></tr>
+
<tr class="tableizer-firstrow"><th>Name</th><th>Manufacturer</th><th></th><th></th></tr>
 +
<tr><td>Phusion Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Phusion High-GC Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Phusion High-GC Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Antarctic Phosphatase Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Antarctic Phosphatase Buffer</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
Line 111: Line 124:
 
</table>
 
</table>
  
−
 
+
<br>
−
<h2> Kits </h2>
+
<br>
−
 
+
−
<table class="tableizer-table">
+
−
<tr class="tableizer-firstrow"><th>Mini-prep</th><th>Qiagen</th><th>Hilden</th><th>Germany</th></tr>
+
−
<tr><td>Mini-prep</td><td>Peqlab</td><td>Erlangen</td><td>Germany</td></tr>
+
−
<tr><td>Mini-prep</td><td>Biozym</td><td>Hessisch Oldendorf</td><td>Germany</td></tr>
+
−
<tr><td>Mini-prep</td><td>Zymo Research</td><td>Freiburg</td><td>Germany</td></tr>
+
−
<tr><td>Midi-prep</td><td>Genomed</td><td>Löhne</td><td>Germany</td></tr>
+
−
<tr><td>Maxi-prep</td><td>Zymo Research</td><td>Freiburg</td><td>Germany</td></tr>
+
−
<tr><td>Gel-Ex</td><td>Qiagen</td><td>Hilden</td><td>Germany</td></tr>
+
−
<tr><td>PCR purification</td><td>Qiagen</td><td>Hilden</td><td>Germany</td></tr>
+
−
<tr><td>DNA concentrator</td><td>Zymo Research</td><td>Freiburg</td><td>Germany</td></tr>
+
−
<tr><td>E. coli S30 Extract System for Linear Templates</td><td>Promega</td><td>&nbsp;</td><td></td></tr>
+
−
</table>
+
  
  
 
<h2> Enzymes </h2>
 
<h2> Enzymes </h2>
 +
<br>
  
 
<table class="tableizer-table">
 
<table class="tableizer-table">
−
<tr class="tableizer-firstrow"><th>Phusion High-Fidelity Polymerase</th><th>NEB (New England Biolabs)</th><th>Ipswitch</th><th>USA</th></tr>
+
<tr class="tableizer-firstrow"><th>Name</th><th>Manufacturer</th><th></th><th></th></tr>
 +
<tr><td>Phusion High-Fidelity Polymerase</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Q5 High-Fidelity Polymerase Master-Mix</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Q5 High-Fidelity Polymerase Master-Mix</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Antarctic Phosphatase</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
 
  <tr><td>Antarctic Phosphatase</td><td>NEB (New England Biolabs)</td><td>Ipswitch</td><td>USA</td></tr>
Line 157: Line 159:
 
</table>
 
</table>
  
 +
<br>
 +
<br>
  
 
<h2> Antibodies </h2>
 
<h2> Antibodies </h2>
 +
<br>
  
 
<table class="tableizer-table">
 
<table class="tableizer-table">
−
<tr class="tableizer-firstrow"><th>anti-tetX antibody :: Goat Tetanus exotoxin polyclonal Antibody </th><th>MyBiosource</th><th>Eching</th><th>Germany</th></tr>
+
<tr class="tableizer-firstrow"><th>Name </th><th>Manufacturer</th><th></th><th></th></tr>
 +
<tr><td>anti-tetX antibody :: Goat Tetanus exotoxin polyclonal Antibody</td><td>MyBiosource</td><td>Eching</td><td>Germany</td></tr>
 
  <tr><td>anti-IgG h+I antibody :: Sheep IgG h+I Antibody</td><td>MyBiosource</td><td>Eching</td><td>Germany</td></tr>
 
  <tr><td>anti-IgG h+I antibody :: Sheep IgG h+I Antibody</td><td>MyBiosource</td><td>Eching</td><td>Germany</td></tr>
 
  <tr><td>Anti-Rabbit IgG (H+L) HRP Conjugate</td><td>Promega</td><td>Madison</td><td>USA</td></tr>
 
  <tr><td>Anti-Rabbit IgG (H+L) HRP Conjugate</td><td>Promega</td><td>Madison</td><td>USA</td></tr>
Line 179: Line 185:
 
</table>
 
</table>
  
−
<h2> Software </h2>
 
  
−
<table class="tableizer-table">
 
−
<tr class="tableizer-firstrow"><th>Geneious</th><th>&nbsp;</th><th>&nbsp;</th><th>PVDF membrane</th><th>Immobilon, 0.45 µm pores</th><th>Roth</th></tr>
 
−
<tr><td>SnapGene</td><td>&nbsp;</td><td>&nbsp;</td><td>Blotting paper</td><td>Whatman</td><td>&nbsp;</td></tr>
 
−
<tr><td>FUSION-Capt, for Western Blot imaging</td><td>Vilber Lourmat</td><td>&nbsp;</td><td>Neodymium magnets</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 
−
<tr><td>digiCamControl</td><td>&nbsp;</td><td>&nbsp;</td><td>Acrylic glass</td><td>&nbsp;</td><td>Formulor.de</td></tr>
 
−
<tr><td>imageJ</td><td>&nbsp;</td><td>&nbsp;</td><td>3W LED (520nm)</td><td>&nbsp;</td><td>Conrad</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>Cooling units</td><td>&nbsp;</td><td>Conrad</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>AC adapter</td><td>&nbsp;</td><td>Conrad</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>microfluidic tubes</td><td>&nbsp;</td><td>Roth</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>2 achromatic lenses (60mm)</td><td>&nbsp;</td><td>Ulbrich (Thorlabs)</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>Canon EOS 550D</td><td>&nbsp;</td><td>Team (R.)</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>iRIf sildes</td><td>&nbsp;</td><td>Biametrics</td></tr>
 
−
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>glass slides</td><td>&nbsp;</td><td>Roth</td></tr>
 
−
</table>
 
  
  

Revision as of 23:43, 18 September 2015

""

Materials


Instruments


DeviceName Manufacturer
ThermocyclerThrillerPeqlabErlangenGermany
 91-D 1200PeqlabErlangenGermany
 Thermomixer comfortEppendorfHamburgGermany
CentrifugeMinispinEppendorfHamburgGermany
 Centrifuge 5424 REppendorfHamburgGermany
 Centrifuge 5804 REppendorfHamburgGermany
 Centrifuge Avanti J-26 XPBeckmanCoulterBreaUSA
Heater/Mixer etcMR Hei StandardHeidolphSchwabachGermany
 Duomax 1030HeidolphSchwabachGermany
 Variable-speed Rock’n roller Model L-202Labinco BVDG BredaNiederlande
 Rotamax 120HeidolphSchwabachGermany
 Innova 44EppendorfHamburgGermany
Vortex Genius 3IKAStaufenGermany
ScaleLE26P-0CESartoriusGöttingenGermany
 440-49 NKernBalingen-FrommernGermany
VacuumpumpME 4C NTVacuubrandWertheimGermany
PCR cyclerGene Amp PCR System 9700Applied BiosystemsWalthamUSA
 Mastercycler epgradient SEppendorfHamburgGermany
PlasmageneratorZEPTODiener electronicsEbhausenGermany
PowersupplyPeqPower 250PeqlabErlangenGermany
BlotterFastblotBiometraGöttingenGermany
Microplate reader Synergy H4 hybridBioTekWinooski USA
Multi-Imager Fusion FX7PeqlabErlangenGermany
French pressFA-078SLM AmincoUrbanaUSA
UV/Vis SpectrophotometerNanoDrop 2000PeqlabErlangenGermany
SDS-PAGE apparatusMighty Small IIHoeferHollistonUSA
OvenUN 30 plusMemmert GmbhSchwabachGermany
Gas filter gunWafergard GNMykron Microelectronics CoBeijingChina


Chemicals


NameManufacturer
Phosphoenolpyruvic acid (monopotassium salt)RocheBaselSwitzerland
Sodium creatine phosphate dibasic tetrahydrateSigmaSt. LouisUSA
Magnesium acetate tetrahydrate (Mg(Oac)2)SigmaSt. LouisUSA
Potassium acetateSigmaSt. LouisUSA
L-Amino Acids KitSigmaSt. LouisUSA
Adenosine 3',5'-cyclic monophosphate (sodium salt)SigmaSt. LouisUSA
L-Glutamic acid potassium salt monohydrateSigmaSt. LouisUSA
Acetic acidRothKarlsruheGermany
DL-DithiothreitolPromegaMadisonUSA
ß-mercaptoethanolRothKarlsruheGermany
PEG4000ThermoFisherWaltham USA
ATP (lithium salt)RocheBaselSchweiz
GTP (lithium salt)RocheBaselSchweiz
CTP (lithium salt)RocheBaselSchweiz
UTP (lithium salt)RocheBaselSchweiz
tRNA from E. coli MRE 600RocheBaselSchweiz
Folinic acid (calcium salt hydrate)SigmaSt. LouisUSA
Acetoacetyl coenzyme A (sodium salt)Santa CruzDallasUSA
D-LuciferinSigmaSt. LouisUSA
Nickel(II) sulfate hexahydrateSigmaSt. LouisUSA
EthanolamineSigmaSt. LouisUSA
1,4-Phenylene diisothiocyanateabcrKarlsruheGermany
3-AminopropyltriethoxysilaneabcrKarlsruheGermany
(3-Glycidoxypropyl)trimethoxysilaneabcrKarlsruheGermany
(3-Chloropropyl)trimethoxysilaneabcrKarlsruheGermany
Na-Bis(carboxymethyl)-L-lysineabcrKarlsruheGermany
Biotinylated Bovine Serum AlbuminThermoFisherWaltham USA
Bovine Serum AlbuminRothKarlsruheGermany
EthanolRothKarlsruheGermany
AcetonRothKarlsruheGermany
2-propanolRothKarlsruheGermany
PyridineSigmaSt. LouisUSA
N,N-DimethylformamidRothKarlsruheGermany
Phosphate buffered salineSigmaSt. LouisUSA


Media and Buffers


NameManufacturer
Phusion BufferNEB (New England Biolabs)IpswitchUSA
Phusion High-GC BufferNEB (New England Biolabs)IpswitchUSA
Antarctic Phosphatase BufferNEB (New England Biolabs)IpswitchUSA
T4 DNA Ligase BufferNEB (New England Biolabs)IpswitchUSA
ISO-BufferNEB (New England Biolabs)IpswitchUSA
CutSmartNEB (New England Biolabs)IpswitchUSA
Buffer 2.1NEB (New England Biolabs)IpswitchUSA
Buffer 3.1NEB (New England Biolabs)IpswitchUSA
Buffer 4.1NEB (New England Biolabs)IpswitchUSA
FastDigest BufferThermo ScientificWaltham USA
FastDigest Green BufferThermo ScientificWaltham USA
LB mediumBIOSSFreiburgGermany
Ampicillin   
Chloramphenicol   
Kanamycin   
Agar   
Agarose   
TAE BufferBIOSSFreiburgGermany
Midori Green AdvanceBiozymHessisch OldendorfGermany
DNA LadderZymo ResearchFreiburgGermany
HEPESRothKarlsruheGermany
KOHRothKarlsruheGermany
TRISRothKarlsruheGermany
Triton X-100SigmaSt. LouisUSA
Tween 20RothKarlsruheGermany
Bovine serum albumin (BSA), blot qualityPromegaMadisonUSA


Enzymes


NameManufacturer
Phusion High-Fidelity PolymeraseNEB (New England Biolabs)IpswitchUSA
Q5 High-Fidelity Polymerase Master-MixNEB (New England Biolabs)IpswitchUSA
Antarctic PhosphataseNEB (New England Biolabs)IpswitchUSA
Blunting EnzymeNEB (New England Biolabs)IpswitchUSA
T4 DNA LigaseNEB (New England Biolabs)IpswitchUSA
Taq DNA LigaseNEB (New England Biolabs)IpswitchUSA
T5 ExonucleaseNEB (New England Biolabs)IpswitchUSA
EcoRINEB (New England Biolabs)IpswitchUSA
PstINEB (New England Biolabs)IpswitchUSA
PstI-HFNEB (New England Biolabs)IpswitchUSA
BamHINEB (New England Biolabs)IpswitchUSA
HindIIINEB (New England Biolabs)IpswitchUSA
AflIINEB (New England Biolabs)IpswitchUSA
Acc65INEB (New England Biolabs)IpswitchUSA
SpeINEB (New England Biolabs)IpswitchUSA
NotINEB (New England Biolabs)IpswitchUSA
XbaINEB (New England Biolabs)IpswitchUSA
XhoINEB (New England Biolabs)IpswitchUSA
BamHI-FDThermo ScientificWaltham USA
HindIII-FDThermo ScientificWaltham USA
BspTI-FDThermo ScientificWaltham USA
Pyruvate Kinase (from rabbit muscle)SigmaSt. LouisUSA
Creatine Phosphokinase (from rabbit muscle)SigmaSt. LouisUSA
Streptavidin Cy5 conjugateThermo ScientificWaltham USA


Antibodies


Name Manufacturer
anti-tetX antibody :: Goat Tetanus exotoxin polyclonal AntibodyMyBiosourceEchingGermany
anti-IgG h+I antibody :: Sheep IgG h+I AntibodyMyBiosourceEchingGermany
Anti-Rabbit IgG (H+L) HRP ConjugatePromegaMadisonUSA
Anti-Mouse IgG (H+L) HRP ConjugatePromegaMadisonUSA
anti-PhiYFP antibodyevrogenMoscowRussia
Rabbit anti-HCV Virus Polyclonal AntibodyAnogenMississaugaCanada
Rabbit anti-HIV-1 P24 Polyclonal AntibodyAnogenMississaugaCanada
HIV-1 p24 antibodyProSci Incorporated PowayUSA
GFP Tag AntibodyAbgentSan DiegoUSA
pAB Rabbit anti-mouse IgGAnogenMississaugaCanada
pAB Rabbit anti-mouse IgG HRP conjugatedAnogenMississaugaCanada
Monoclonal neutralizing anti-HIV-1 gp41DendriticsLyonFrance
Monoclonal neutralizing anti-HIV-1 gp120/160DendriticsLyonFrance
Anti-Clostridium tetani toxoid (Tetanus toxoid)BioPortoHellerupDenmark
anti-GFP antibody, monoclonal, from goatRocklandLimerickUSA
anti-Goat HRP-labelled antibody, polyclonal, f. rabbitRocklandLimerickUSA