Difference between revisions of "Team:Freiburg/Materials"

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A list of plasmids we used/constructed during our project can be found <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Labjournals/Plasmids">here.</a>
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<h1> Materials </h1>
 
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<h1> Materials </h1>
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A list of plasmids we used/constructed during our project can be found <a class="wikilink1" href="https://2015.igem.org/Team:Freiburg/Labjournals/Plasmids">here.</a>
 
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<h2> Instruments </h2>
 
<h2> Instruments </h2>

Revision as of 23:54, 18 September 2015

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Materials



A list of plasmids we used/constructed during our project can be found here.

Instruments

DeviceName Manufacturer
ThermocyclerThrillerPeqlabErlangenGermany
 91-D 1200PeqlabErlangenGermany
 Thermomixer comfortEppendorfHamburgGermany
CentrifugeMinispinEppendorfHamburgGermany
 Centrifuge 5424 REppendorfHamburgGermany
 Centrifuge 5804 REppendorfHamburgGermany
 Centrifuge Avanti J-26 XPBeckmanCoulterBreaUSA
Heater/Mixer etcMR Hei StandardHeidolphSchwabachGermany
 Duomax 1030HeidolphSchwabachGermany
 Variable-speed Rock’n roller Model L-202Labinco BVDG BredaNiederlande
 Rotamax 120HeidolphSchwabachGermany
 Innova 44EppendorfHamburgGermany
Vortex Genius 3IKAStaufenGermany
ScaleLE26P-0CESartoriusGöttingenGermany
 440-49 NKernBalingen-FrommernGermany
VacuumpumpME 4C NTVacuubrandWertheimGermany
PCR cyclerGene Amp PCR System 9700Applied BiosystemsWalthamUSA
 Mastercycler epgradient SEppendorfHamburgGermany
PlasmageneratorZEPTODiener electronicsEbhausenGermany
PowersupplyPeqPower 250PeqlabErlangenGermany
BlotterFastblotBiometraGöttingenGermany
Microplate reader Synergy H4 hybridBioTekWinooski USA
Multi-Imager Fusion FX7PeqlabErlangenGermany
French pressFA-078SLM AmincoUrbanaUSA
UV/Vis SpectrophotometerNanoDrop 2000PeqlabErlangenGermany
SDS-PAGE apparatusMighty Small IIHoeferHollistonUSA
OvenUN 30 plusMemmert GmbhSchwabachGermany
Gas filter gunWafergard GNMykron Microelectronics CoBeijingChina


Chemicals


NameManufacturer
Phosphoenolpyruvic acid (monopotassium salt)RocheBaselSwitzerland
Sodium creatine phosphate dibasic tetrahydrateSigmaSt. LouisUSA
Magnesium acetate tetrahydrate (Mg(Oac)2)SigmaSt. LouisUSA
Potassium acetateSigmaSt. LouisUSA
L-Amino Acids KitSigmaSt. LouisUSA
Adenosine 3',5'-cyclic monophosphate (sodium salt)SigmaSt. LouisUSA
L-Glutamic acid potassium salt monohydrateSigmaSt. LouisUSA
Acetic acidRothKarlsruheGermany
DL-DithiothreitolPromegaMadisonUSA
ß-mercaptoethanolRothKarlsruheGermany
PEG4000ThermoFisherWaltham USA
ATP (lithium salt)RocheBaselSchweiz
GTP (lithium salt)RocheBaselSchweiz
CTP (lithium salt)RocheBaselSchweiz
UTP (lithium salt)RocheBaselSchweiz
tRNA from E. coli MRE 600RocheBaselSchweiz
Folinic acid (calcium salt hydrate)SigmaSt. LouisUSA
Acetoacetyl coenzyme A (sodium salt)Santa CruzDallasUSA
D-LuciferinSigmaSt. LouisUSA
Nickel(II) sulfate hexahydrateSigmaSt. LouisUSA
EthanolamineSigmaSt. LouisUSA
1,4-Phenylene diisothiocyanateabcrKarlsruheGermany
3-AminopropyltriethoxysilaneabcrKarlsruheGermany
(3-Glycidoxypropyl)trimethoxysilaneabcrKarlsruheGermany
(3-Chloropropyl)trimethoxysilaneabcrKarlsruheGermany
Na-Bis(carboxymethyl)-L-lysineabcrKarlsruheGermany
Biotinylated Bovine Serum AlbuminThermoFisherWaltham USA
Bovine Serum AlbuminRothKarlsruheGermany
EthanolRothKarlsruheGermany
AcetonRothKarlsruheGermany
2-propanolRothKarlsruheGermany
PyridineSigmaSt. LouisUSA
N,N-DimethylformamidRothKarlsruheGermany
Phosphate buffered salineSigmaSt. LouisUSA


Media and Buffers


NameManufacturer
Phusion BufferNEB (New England Biolabs)IpswitchUSA
Phusion High-GC BufferNEB (New England Biolabs)IpswitchUSA
Antarctic Phosphatase BufferNEB (New England Biolabs)IpswitchUSA
T4 DNA Ligase BufferNEB (New England Biolabs)IpswitchUSA
ISO-BufferNEB (New England Biolabs)IpswitchUSA
CutSmartNEB (New England Biolabs)IpswitchUSA
Buffer 2.1NEB (New England Biolabs)IpswitchUSA
Buffer 3.1NEB (New England Biolabs)IpswitchUSA
Buffer 4.1NEB (New England Biolabs)IpswitchUSA
FastDigest BufferThermo ScientificWaltham USA
FastDigest Green BufferThermo ScientificWaltham USA
LB mediumBIOSSFreiburgGermany
Ampicillin   
Chloramphenicol   
Kanamycin   
Agar   
Agarose   
TAE BufferBIOSSFreiburgGermany
Midori Green AdvanceBiozymHessisch OldendorfGermany
DNA LadderZymo ResearchFreiburgGermany
HEPESRothKarlsruheGermany
KOHRothKarlsruheGermany
TRISRothKarlsruheGermany
Triton X-100SigmaSt. LouisUSA
Tween 20RothKarlsruheGermany
Bovine serum albumin (BSA), blot qualityPromegaMadisonUSA


Enzymes


NameManufacturer
Phusion High-Fidelity PolymeraseNEB (New England Biolabs)IpswitchUSA
Q5 High-Fidelity Polymerase Master-MixNEB (New England Biolabs)IpswitchUSA
Antarctic PhosphataseNEB (New England Biolabs)IpswitchUSA
Blunting EnzymeNEB (New England Biolabs)IpswitchUSA
T4 DNA LigaseNEB (New England Biolabs)IpswitchUSA
Taq DNA LigaseNEB (New England Biolabs)IpswitchUSA
T5 ExonucleaseNEB (New England Biolabs)IpswitchUSA
EcoRINEB (New England Biolabs)IpswitchUSA
PstINEB (New England Biolabs)IpswitchUSA
PstI-HFNEB (New England Biolabs)IpswitchUSA
BamHINEB (New England Biolabs)IpswitchUSA
HindIIINEB (New England Biolabs)IpswitchUSA
AflIINEB (New England Biolabs)IpswitchUSA
Acc65INEB (New England Biolabs)IpswitchUSA
SpeINEB (New England Biolabs)IpswitchUSA
NotINEB (New England Biolabs)IpswitchUSA
XbaINEB (New England Biolabs)IpswitchUSA
XhoINEB (New England Biolabs)IpswitchUSA
BamHI-FDThermo ScientificWaltham USA
HindIII-FDThermo ScientificWaltham USA
BspTI-FDThermo ScientificWaltham USA
Pyruvate Kinase (from rabbit muscle)SigmaSt. LouisUSA
Creatine Phosphokinase (from rabbit muscle)SigmaSt. LouisUSA
Streptavidin Cy5 conjugateThermo ScientificWaltham USA


Antibodies


Name Manufacturer
anti-tetX antibody :: Goat Tetanus exotoxin polyclonal AntibodyMyBiosourceEchingGermany
anti-IgG h+I antibody :: Sheep IgG h+I AntibodyMyBiosourceEchingGermany
Anti-Rabbit IgG (H+L) HRP ConjugatePromegaMadisonUSA
Anti-Mouse IgG (H+L) HRP ConjugatePromegaMadisonUSA
anti-PhiYFP antibodyevrogenMoscowRussia
Rabbit anti-HCV Virus Polyclonal AntibodyAnogenMississaugaCanada
Rabbit anti-HIV-1 P24 Polyclonal AntibodyAnogenMississaugaCanada
HIV-1 p24 antibodyProSci Incorporated PowayUSA
GFP Tag AntibodyAbgentSan DiegoUSA
pAB Rabbit anti-mouse IgGAnogenMississaugaCanada
pAB Rabbit anti-mouse IgG HRP conjugatedAnogenMississaugaCanada
Monoclonal neutralizing anti-HIV-1 gp41DendriticsLyonFrance
Monoclonal neutralizing anti-HIV-1 gp120/160DendriticsLyonFrance
Anti-Clostridium tetani toxoid (Tetanus toxoid)BioPortoHellerupDenmark
anti-GFP antibody, monoclonal, from goatRocklandLimerickUSA
anti-Goat HRP-labelled antibody, polyclonal, f. rabbitRocklandLimerickUSA