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| | + | For few years, influenza H7N9 has been a big threat for public health in China, yet traditional vaccination is not so efficient to it. In order to tackle this problem, our team investigated another approach to make an effective and promising cure for influenza H7N9. In our project, we clone HA,NA,M1 genes into a pCDNA5.1 FRT vector and co-transfect it into 293T cells to produce virus-like particles(VLPs). Then we purify VLPs by ultracentrifugation and inject into mice. Finally, we detect IgG by ELISA. Compared with traditional live attenuated vaccine and inactivated vaccine, H7N9 VLPs possess advantage in effectiveness towards the elderly and mass-production abilities. Plus, compared with previous VLPs made in expression systems such as E.coli and yeast, H7N9 VPLs are made mammalian cells. This can make VLPs be cut effectively, be formed into more natural conformations, and be increased in antigenicity and morphology.《/br> |
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| − | <p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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| − | <h5>What should this page contain?</h5>
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| − | <li> A clear and concise description of your project.</li>
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| − | <li>A detailed explanation of why your team chose to work on this particular project.</li>
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| − | <li>References and sources to document your research.</li>
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| − | <h4>Advice on writing your Project Description</h4>
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| − | We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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| − | Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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| − | <h4>References</h4>
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| − | <p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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| − | <h4>Inspiration</h4>
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| − | <p>See how other teams have described and presented their projects: </p>
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| − | <li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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| − | <li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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| − | <li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Latest revision as of 02:00, 19 September 2015
Indian Institute of Science Education and Research (IISER), Pune, India
Project Description
For few years, influenza H7N9 has been a big threat for public health in China, yet traditional vaccination is not so efficient to it. In order to tackle this problem, our team investigated another approach to make an effective and promising cure for influenza H7N9. In our project, we clone HA,NA,M1 genes into a pCDNA5.1 FRT vector and co-transfect it into 293T cells to produce virus-like particles(VLPs). Then we purify VLPs by ultracentrifugation and inject into mice. Finally, we detect IgG by ELISA. Compared with traditional live attenuated vaccine and inactivated vaccine, H7N9 VLPs possess advantage in effectiveness towards the elderly and mass-production abilities. Plus, compared with previous VLPs made in expression systems such as E.coli and yeast, H7N9 VPLs are made mammalian cells. This can make VLPs be cut effectively, be formed into more natural conformations, and be increased in antigenicity and morphology.《/br>