Difference between revisions of "Team:Edinburgh/Composite Part"

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                     <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne">1. What is HTML?</a>
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                     <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne">mRFP driven by LacI promoter (BBa_K1615090)</a>
  
 
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                 <div class="panel-body">
 
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                     <p>HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. <a href="http://www.tutorialrepublic.com/html-tutorial/" target="_blank">Learn more.</a></p>
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                     <p>mRFP (BBa_K1615089) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the <i>mRFP</i> gene to express mRFP when induced with IPTG.
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</p>
  
 
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                     <a data-toggle="collapse" data-parent="#accordion" href="#collapseTwo">2. What is Bootstrap?</a>
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                     <a data-toggle="collapse" data-parent="#accordion" href="#collapseTwo">CBDcenA+Linker fused to mRFP in RFC25 (BBa_K1615091)</a>
  
 
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                 <div class="panel-body">
 
                 <div class="panel-body">
  
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                     <p>Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. <a href="http://www.tutorialrepublic.com/twitter-bootstrap-tutorial/" target="_blank">Learn more.</a></p>
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                     <p>mRFP (BBa_K1615089) fused to the C terminal of CBDcenA + C-terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
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<br>
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<img src="https://static.igem.org/mediawiki/2015/2/20/CBD_RFP_folding.jpeg">
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<br>
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To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator.
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</p>
  
 
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                     <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">3. What is CSS?</a>
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                     <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">mRFP fused to CBDcenA+Linker in RFC25 (BBa_K16150092)</a>
  
 
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                     <p>CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. <a href="http://www.tutorialrepublic.com/css-tutorial/" target="_blank">Learn more.</a></p>
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                     <p>mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
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</p>
  
 
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      <footer>
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        <p class="pull-right"><a href="#">Back to top</a></p>
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        <p>&copy; 2015 EdiGEM &middot; <a href="#">Privacy</a> &middot; <a href="#">Terms</a></p>
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      </footer>
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                <h4 class="panel-title">
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                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">mRFP fused to CBDcenA+Linker in RFC25 (BBa_K16150092)</a>
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            </div>
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            <div id="collapseThree" class="panel-collapse collapse">
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                <div class="panel-body">
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                    <p>mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2015/2/20/CBD_RFP_folding.jpeg">
 +
<br>
 +
<br>
 +
We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.
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<br>
 +
<br>
 +
We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.
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</p>
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</div>
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        <div class="panel panel-default">
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            <div class="panel-heading">
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                <h4 class="panel-title">
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                    <a data-toggle="collapse" data-parent="#accordion" href="#collapseThree">Linker +dCBD fused to mRFP in RFC25 (BBa_K1615093)
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</a>
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                </h4>
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            </div>
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            <div id="collapseThree" class="panel-collapse collapse">
 +
 +
                <div class="panel-body">
 +
 +
                    <p>mRFP (BBa_K1615089) fused to the C terminal of dCBD + N terminal linker (BBa_K1321340). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2015/2/20/CBD_RFP_folding.jpeg">
 +
<br>
 +
To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator.
 +
</p>
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                </div>
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            </div>
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    </div>
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</div>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 03:45, 19 September 2015

Composite Parts

Heroin Esterase

1. What is HTML?

HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. Learn more.

2. What is Bootstrap?

Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. Learn more.

3. What is CSS?

CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. Learn more.

Morphine-6-Dehydrogenase

1. What is HTML?

HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. Learn more.

2. What is Bootstrap?

Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. Learn more.

3. What is CSS?

CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. Learn more.

Monoamine Oxidase A

1. What is HTML?

HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. Learn more.

2. What is Bootstrap?

Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. Learn more.

3. What is CSS?

CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. Learn more.

Laccase

1. What is HTML?

HTML stands for HyperText Markup Language. HTML is the main markup language for describing the structure of Web pages. Learn more.

2. What is Bootstrap?

Bootstrap is a powerful front-end framework for faster and easier web development. It is a collection of CSS and HTML conventions. Learn more.

3. What is CSS?

CSS stands for Cascading Style Sheet. CSS allows you to specify various style properties for a given HTML element such as colors, backgrounds, fonts etc. Learn more.

mRFP

mRFP driven by LacI promoter (BBa_K1615090)

mRFP (BBa_K1615089) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the mRFP gene to express mRFP when induced with IPTG.

CBDcenA+Linker fused to mRFP in RFC25 (BBa_K1615091)

mRFP (BBa_K1615089) fused to the C terminal of CBDcenA + C-terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator.

mRFP fused to CBDcenA+Linker in RFC25 (BBa_K16150092)

mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.

mRFP fused to CBDcenA+Linker in RFC25 (BBa_K16150092)

mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.



We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.

We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.

Linker +dCBD fused to mRFP in RFC25 (BBa_K1615093)

mRFP (BBa_K1615089) fused to the C terminal of dCBD + N terminal linker (BBa_K1321340). If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.


To test the RFP folding when fused to a CBD we placed 200µL of crude cell lysate in a 96 well plate and observed the fluorescence under a blue light transilluminator.