Difference between revisions of "Team:Brasil-USP/capa"

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     !-- Brasil-USP INTRODUCTION
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      <div class="content">
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==============================================
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<div id="Br"><a href=""><img src="https://static.igem.org/mediawiki/2015/e/e0/Team-Brasil-USP_CapaTopo_small.png" height="45px" /></a></div>
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       -->  
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<div id="logo"><a href=""> iGEM Brasil-USP </a></div>
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<nav id="nav">
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  <ul>
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    <li><a href="#capa" class="active" title="capa" >Home</a></li>
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    <li><a href="#Intro" title="project">The Project</a></li>
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    <li><a href="#wiki" title="wiki">Our Progress</a></li>
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    <li><a href="#sponsors" title="sponsors">The Sponsors</a></li>
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    <li><a href="#team" title="team">The Team</a></li>
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  </ul>
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</nav>
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       </div>
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    </header>
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     <div id="capa">
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     <div class="container marketing">
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       <div class="content">
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<img class="igem" align="center" src="https://static.igem.org/mediawiki/2015/f/f7/Team-Brasil-USP_CapaMainsymbol.png" />
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      <!-- START THE FEATURETTES -->
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<h2>Degradation of</h2>
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<h1>Natural Rubber</h1>
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       <div class="row featurette">
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<div id="square">
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<div class="container">
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  <h3>Team Brasil-USP</h3>
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          <div class="col-md-7">
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            <h2 class="featurette-heading center">
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      Brasil-USP team's 2015 project is to develop an innovative
 +
              and sustainable solution for a significant environmental
 +
              problem: <br /><span class="TextoVerde">accumulation of
 +
waste tires</span>
 +
    </h2>
 +
            <p class="lead">
 +
      Current recycling techniques demand high energy
 +
      consumption, and
 +
      are <span class="TextoVermelho"><b>completely overwhelmed
 +
  by the annual amount of rubber waste</b></span>. In
 +
      addition, natural rubber degradation may take up to 1000
 +
      years.
 +
    </p>
 +
          </div>
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          <div class="col-md-5">
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    <div id="videoCover_">
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              <video width="450" id="videoCover" autoplay muted loop>
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<source src="https://static.igem.org/mediawiki/2015/1/1d/Team-Brasil-USP_VideoPneus.webm" type="video/webm" />
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<!--<source src="https://static.igem.org/mediawiki/2015/b/bd/Team-Brasil-USP_VideoPneus.mp4" type="video/mp4" />
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  -->               <source src="https://static.igem.org/mediawiki/2015/8/87/Team-Brasil-USP_VideoPneus.mov" type="video/mov">
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  Your browser does not support the video element.
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              </video>
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            </div>
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          </div>
 
</div>
 
</div>
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       </div>  
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       </div>
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    </div>  
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 +
      <hr class="featurette-divider">
  
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      <div class="row meio featurette">
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    <div id="Intro">
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<div class="container">
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      <div class="content" >
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          <div class="col-md-7 col-md-push-5">
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        <div id="videoCover_">
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             <h2 class="featurette-heading">
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             <video width="450" id="videoCover" autoplay muted loop>
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      We aim to accelerate this process by genetically
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              <source src="https://static.igem.org/mediawiki/2015/1/1d/Team-Brasil-USP_VideoPneus.webm" />
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      engineering microorganisms to express and secrete two
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              Your browser does not support the video element.
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      crucial enzymes for the degradation of natural
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            </video>
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      rubber: <span class="TextoAzul"><b>RoxA</b></span>
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        </div>
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      (Rubber oxygenase)
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        <!--<script>
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      and <span class="TextoAzul"><b>Lcp</b></span> (Latex
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            document.getElementById("videoCover").width=document.body.offsetWidth;
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      clearing protein).
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        </script>-->
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    </h2>
 +
           
 +
    <p class="lead">
 +
      Tires and several other products, however, are composed
 +
      by vulcanized rubber, which ensures its durability and
 +
      increase its elasticity and strength. In this regard,
 +
      for vulcanized rubber degradation, the project may
 +
      include a pre-treatment, which uses Acidithiobacillus
 +
      ferrooxidans, bacteria that naturally devulcanizes
 +
      rubber. Both of these processes will be scaled up in
 +
      bioreactors.
 +
    </p>
 +
          </div>
 +
          <div class="col-md-5 col-md-pull-7">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/4b/Team-Brasil-USP_CapaDesenho.png" height="450"/>
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          </div>
 +
</div>
 +
      </div>
  
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        <div id="colunadireita">
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      <hr class="featurette-divider">
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        <h3 class="title">
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            Brasil-USP team's 2015 project is to develop an
+
−
            innovative and sustainable solution for a significant environmental problem:
+
−
            <br /><span class="TextoVerde">accumulation of waste tires</span>.
+
−
        </h3>
+
−
+
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        <br />
+
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<h3>Current recycling techniques demand high energy consumption, and are <span class="TextoVermelho"><b>completely overwhelmed by the annual amount of rubber waste</b></span>. In addition, natural rubber degradation may take up to 1000 years.</h3>
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        </div>
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        <div class="downarrow">
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            <a href="#project2"><img src="https://static.igem.org/mediawiki/2015/0/09/Team-Brasil-USP_CapaDownArrow.png" /></a>
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        </div>
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       </div>  
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       <div class="row featurette last">
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    </div>  
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<h1>Both of these processes will be scaled up in bioreactors!</h1>
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<img src="https://static.igem.org/mediawiki/2015/9/9f/Team-Brasil-USP_CapaReator.png" />
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    <div id="project2">
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      </div>
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       <div class="content" >
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<div id="colunaesquerda">
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      <hr class="featurette-divider">
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            <img src="https://static.igem.org/mediawiki/2015/4/4b/Team-Brasil-USP_CapaDesenho.png" height="450"/>
+
     
 +
     
 +
       <div class="row threecolumns">
 +
        <div class="col-lg-4">
 +
          <img class="img-circle" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Generic placeholder image" width="140" height="140">
 +
  <div class="board">
 +
            <h2>Our main circuit</h2>
 +
            <p>
 +
      The main idea behind our project is based on the gene
 +
      expression of a very simple circuit, followed by the
 +
      exportation of Lcp e RoxA enzymes. Then, both interact
 +
      with the polymers in the natural rubber and generate
 +
      several products. One of them is ODTD, which can then be
 +
      turned into high value fuel. Through experiments and
 +
      modeling, we have investigated the main features of our
 +
      system and optimized it for a bioreactor.
 +
    </p>
 +
    <p>
 +
      <a class="btn btn-primary" href="#" role="button">Learn
 +
more &raquo;</a>
 +
    </p>
 +
          </div>
 
</div>
 
</div>
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<div id="colunadireita">
+
−
            <br /><br /><br />
+
        <div class="col-lg-4">
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    <h3>We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: <span class="TextoAzul"><b>RoxA</b></span> (Rubber oxygenase) and <span class="TextoAzul"><b>Lcp</b></span> (Latex clearing protein).</h3>
+
          <img class="img-circle" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Generic placeholder image" width="140" height="140">
−
              
+
  <div class="board">
−
    <h3>Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.</h3>  
+
            <h2>Our Parts</h2>
−
        </div>
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             <p>
−
        <div class="downarrow">
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      We've submitted two different parts and characterized
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            <a href="#project"><img src="https://static.igem.org/mediawiki/2015/0/09/Team-Brasil-USP_CapaDownArrow.png" /></a>
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      the main circuit that should be used in the degradation
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        </div>
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      of natural rubber. All these parts involve Lcp and RoxA
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      </div>  
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      production with Rhamnose promoter. All these parts
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    </div>  
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      involve Lcp and RoxA production and exportation with
−
 
+
      Rhamnose promoter. Especially to better understand/model
−
    <div id="project">
+
      our kill switch mechanism (highly based on TetR
−
      <div class="content" >
+
      inhibition), we have characterized a simple circuit
−
        <h3 class="title" style="color: #333;">Both of these processes will be scaled up in bioreactors!</h3>
+
      where TetR inhibits Gfp production. This gave us
−
        <img src="https://static.igem.org/mediawiki/2015/9/9f/Team-Brasil-USP_CapaReator.png" height="400" style="display:block; margin: 0 auto 0 auto;" />
+
      important information on the timing balance and promoter
−
      </div>  
+
      strength of our main circuit.
−
        <div class="downarrow">
+
    </p>
−
            <a href="#project2"><img src="https://static.igem.org/mediawiki/2015/0/09/Team-Brasil-USP_CapaDownArrow.png" /></a>
+
            <p>
−
        </div>
+
      <a class="btn btn-primary" href="#" role="button">Learn
−
    </div>  
+
more &raquo;</a>
−
 
+
    </p>
−
  <div id="wiki">
+
          </div>
−
    <div class="content">
+
</div><!-- /.col-lg-4 -->
−
      <h1>Very soon we will share our progress and results!</h1>
+
        <div class="col-lg-4">
−
      <img src="https://static.igem.org/mediawiki/2015/f/f4/Team-Brasil-USP_CapaFotosTime.jpg" />
+
          <img class="img-circle" src="data:image/gif;base64,R0lGODlhAQABAIAAAHd3dwAAACH5BAAAAAAALAAAAAABAAEAAAICRAEAOw==" alt="Generic placeholder image" width="140" height="140">
−
    </div>  
+
  <div class="board">
−
  </div>  
+
            <h2>Entrepreneurship</h2>
−
 
+
            <p>
−
  <div id="sponsors">
+
      Given the high potential for an industrial application,
−
    <div class="content">
+
      we have investigated possible business plans that could
−
      <h1>These are our sponsors</h1>
+
      turn our project into a competitive startup. As a first
−
      <img src="https://static.igem.org/mediawiki/2015/4/4c/Team-Brasil-USP_CapaSponsors.png" />
+
      step, we have met with warehouses where waste tires are
−
    </div>  
+
      treated and discarded to both promote our ideas and
−
  </div>  
+
      learn more on how to implement our system into their
−
 
+
      reality. Several chemical processes involved in our
−
 
+
      project are indeed complex, demanding some extra sfatety
−
  <div id="team">
+
      care.
−
    <div class="content">
+
    </p>
−
<h1>
+
            <p>
−
    We are students and post docs from<br />
+
      <a class="btn btn-primary" href="#" role="button">Learn
−
    University of São Paulo, Brazil
+
more &raquo;</a>
−
</h1>
+
          </div>
−
<h2>
+
  </p>
−
    Learn more about our team!
+
        </div><!-- /.col-lg-4 -->
−
</h2>
+
      </div><!-- /.row -->
−
    </div>  
+
 
−
  </div>  
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+
 
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  <!----- SLIDES END --->
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      <hr class="featurette-divider">
−
 
+
     
−
</html>
+
      <div class="sponsors">
 +
<div class="container">
 +
          <h1>We thank our sponsors for all support!</h1>
 +
 
 +
  <img src="https://static.igem.org/mediawiki/2015/4/4c/Team-Brasil-USP_CapaSponsors.png" />
 +
 
 +
          <p><a class="btn btn-primary btn-lg" href="#" role="button">Learn more &raquo;</a></p>
 +
</div>
 +
      </div>
 +
     
 +
      <hr class="featurette-divider">
 +
 
 +
 
 +
    </div><!-- /.container -->
 +
 
 +
   
 +
    <div id="team">
 +
      <div class="container">
 +
        <h1>And this is Brasil-USP Team, from University of São Paulo!</h1>
 +
        <p><a class="btn btn-primary btn-lg" href="#" role="button">Learn more &raquo;</a></p>
 +
      </div>
 +
    </div>
  
  
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{{:Team:Brasil-USP/Templates/CoverFoot}}
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{{:Team:Brasil-USP/Templates/Foot}}

Revision as of 05:01, 23 July 2015

Degradation of Natural Rubber

Team Brasil-USP

!-- Brasil-USP INTRODUCTION ============================================== -->

Brasil-USP team's 2015 project is to develop an innovative and sustainable solution for a significant environmental problem:
accumulation of waste tires

Current recycling techniques demand high energy consumption, and are completely overwhelmed by the annual amount of rubber waste. In addition, natural rubber degradation may take up to 1000 years.

We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: RoxA (Rubber oxygenase) and Lcp (Latex clearing protein).

Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.

Both of these processes will be scaled up in bioreactors!

Generic placeholder image

Our main circuit

The main idea behind our project is based on the gene expression of a very simple circuit, followed by the exportation of Lcp e RoxA enzymes. Then, both interact with the polymers in the natural rubber and generate several products. One of them is ODTD, which can then be turned into high value fuel. Through experiments and modeling, we have investigated the main features of our system and optimized it for a bioreactor.

Learn more »

Generic placeholder image

Our Parts

We've submitted two different parts and characterized the main circuit that should be used in the degradation of natural rubber. All these parts involve Lcp and RoxA production with Rhamnose promoter. All these parts involve Lcp and RoxA production and exportation with Rhamnose promoter. Especially to better understand/model our kill switch mechanism (highly based on TetR inhibition), we have characterized a simple circuit where TetR inhibits Gfp production. This gave us important information on the timing balance and promoter strength of our main circuit.

Learn more »

Generic placeholder image

Entrepreneurship

Given the high potential for an industrial application, we have investigated possible business plans that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Several chemical processes involved in our project are indeed complex, demanding some extra sfatety care.

Learn more »

We thank our sponsors for all support!

Learn more »

And this is Brasil-USP Team, from University of São Paulo!

Learn more »

{{:Team:Brasil-USP/Templates/Foot}}