Difference between revisions of "Team:British Columbia/Notebook/Protocols"
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| − | To amplify an insert for cloning, using high-fidelity phusion polymerase. | + | <p>To amplify an insert for cloning, using high-fidelity phusion polymerase.</p> |
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| − | Before primers can be used for PCR they need to be resuspended and diluted. | + | <p>Before primers can be used for PCR they need to be resuspended and diluted.</p> |
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Revision as of 22:53, 31 March 2015
Protocols: Use the categories on the left to find the protocol you need:
To amplify an insert for cloning, using high-fidelity phusion polymerase.
Colony PCR is commonly used to screen for the presence of a insert in a plasmid during cloning.
Before primers can be used for PCR they need to be resuspended and diluted.
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