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Revision as of 18:17, 28 July 2015
Under Construction.
The CMU iGEM team did a lot this summer from building a light to playing with an Arduino to improving an estrogen sensor. Luckily, we tracked everything in our handy-dandy notebook.
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Week 1
May 26th, 2015
- First day of work!
- Familiarized ourselves with the lab.
- Made LB (protocol) & poured plates (protocol).
The light fluoresces! We have transformants & isolated proteins.
Week 1
5.26.15
Week 2
Week 3
Week 4
Week 5
Estrogen Sensor:
- tried newly transformed cells, all taken from the same plate
- tried to get similar-sized colonies tested the following amounts of estrogen: 100 uM, 20 uM, 10 uM, 1 uM, 100 nM, 10 nM, 1 nM, 0 nM got very varied and inconsistent results no pattern between amount of estrogen added & reading high standard deviation think that there is a problem with the transformation will try another transformation
la la la
yo this is cool