Difference between revisions of "Team:UMBC-Maryland/Experiments"

Revision as of 05:17, 14 August 2015

Electrophoresis buffer contents:

10 mL 50x TAE

490 mL deionized water

50 μL Bullseye DNA SafeStain

Contents of 1% Agarose gel:

1g Agarose

100 mL Electrophoresis buffer

Mix agarose and electrophoresis buffer and microwave for 1.5 minutes.

When the solution is sufficiently cool, add 6 µL of EtBr.

Pour the solution into a bed with a comb levely placed within. Clear the bubbles from the surface and wait for the gel to polymerize.

Mix loading dye and sample in a 1:5 ratio and insert samples into wells. Place 10 µL of DNA ladder into the first well.

@@ Line 244: / Line 244: @@
 <h5>1% Agarose Gel</h5>
-<p> Electrophoresis buffer contents:
+<p>
-mL 50x TAE
+Electrophoresis buffer contents:
-mL deionized water
+<li>10 mL 50x TAE</li>
-μL Bullseye DNA SafeStain
+<li>490 mL deionized water</li>
+<li>50 μL Bullseye DNA SafeStain</li>
+<br>
 Contents of 1% Agarose gel:
-g Agarose
+<li>1g Agarose</li>
-mL Electrophoresis buffer
+<li>100 mL Electrophoresis buffer</li>
+<br>
-Mix agarose and electrophoresis buffer and microwave for 1.5 minutes.
+<li>Mix agarose and electrophoresis buffer and microwave for 1.5 minutes.</li>
-When the solution is sufficiently cool, add 6 µL of EtBr.
+<li>When the solution is sufficiently cool, add 6 µL of EtBr.</li>
-Pour the solution into a bed with a comb levely placed within. Clear the bubbles from the surface and wait for the gel to polymerize.
+<li>Pour the solution into a bed with a comb levely placed within. Clear the bubbles from the surface and wait for the gel to polymerize.</li>
-Mix loading dye and sample in a 1:5 ratio and insert samples into wells. Place 10 µL of DNA ladder into the first well. </p>
+<li>Mix loading dye and sample in a 1:5 ratio and insert samples into wells. Place 10 µL of DNA ladder into the first well.</li>  </p>
 <h5>What should this page contain?</h5>