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E.coli genotypes

We are using E.coli strains for overexpression of our target proteins. E. coli is suitable for expression of a wide range of heterologous proteins. Our aim was the enrichment of active, soluble recombinant protein. Therefore, we used different E.coli strains and adapted different expression temperatures, durations and IPTG concentrations to get as much soluble target protein as possible.

Of course, many challenges can arise during expression of recombinant proteins. That's why most expression strains have a few mutations in common to obtain a high yield of expressed proteins.

Expression strains often harbor mutations that make them deficient in outer membrane protease VII and delete the Ion protease. Both of this reduces proteolysis of the expressed recombinant proteins. Additionally the native restriction/methylation system is often inactivated.

Below we indtroduce the bacterial strains we used for protein overexpression: