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Biobricks

Since our project involved expression of many antigens we decided to share those antigen-sequences with the iGEM community. To do this we used the codon optimization tool from Integrated DNA Technologies to improve most sequences for expression in E. coli. We also removed all restriction sites that are not allowed in RFC[10], so that all sequences are compatible with the submission vector pSB1C3. We obtained most of the sequences via paper research and we would like to give special thanks to the group of Prof. Dr. Michael Hust (TU Braunschweig) who provided us with expression plasmids for the Salmonella Typhimurium antigen and a corresponding single chain variable fragment. When we started working with our first plasmids for this project, we decided to use a specific nomenclature. Every plasmid name starts with “pIG15” which is short for “plasmid igem 2015”. According to this, we named the plasmids containing our biobricks in the shipping backbone pRIG15 (“pRIG” as in “brick”)

biobrick	short description	detailed desription
BBa_K1621000	Rubella Virus specific antigenic epitopes derived from glycoprotein E1	pRIG15_6
BBa_K1621001	Varicella Zoster Virus specific antigenic epitopes derived from glycoprotein E	pRIG15_7
BBA_K1621002	Herpes Simplex specific antigenic epitopes derived from glycoprotein G	pRIG15_8
BBa_K1621003	Clostridium tetani specific antigenic epitopes derived from tetnus neurotoxin (TeNT_Hc)	pRIG15_11
BBa_K1621004	Human Immunodeficiency Virus specific antigenic epitopes derived from a polyprotein called gag/tat/pol/env	pRIG15_17
BBa_K1621005	Treponema pallidum specific antigenic peptides derived from bacterioferritin (TpF1)	pRIG15_18
BBa_K1621006	Salmonella Typhimurium specific antigenic protein (DHAD)	pRIG15_15
BBa_K1621007	scFv binding specifically to the Salmonella Typhimurium derived antigen	pRIG15_13
BBa_K1621009	Standardized plasmid backbone optimized for protein overexpression	pOP

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