Team:Austin UTexas/Project/Spring Data
--this is a first draft--
Spring 2015 Data Set
Plasmid Construction and Transformation
Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:
BBa_E0020+BBa_K608007
BBa_EE030+BBa_K608006
BBa_E0020+BBa_K608003
BBa_E0030+BBa_K608007
BBa_K592101+BBa_K608006
BBa_K592100+BBa_K608004
BBa_K592101+BBa_K608002
BBa_K864100+BBa_K608006
BBa_E0030+BBa_K314100
BBa_E0020+BBa_K608002
BBa_K864100+BBa_K608002
BBa_E0030+BBa_K314100
BBa_K592101+BBa_K608007
discuss what these BioBricks are
plasmids transformed by electroporation into pSB1c3
Fluorescence Readings
relative fluorescence units
96 well plate
fluorescence data summary
summary figure(?)
Observations and Discussion
insert mutations in SFYFP
EYFP, CYFP stable
what contributes to stability
what does this mean about what sequences are stable</br>
next steps