Team:NYU Shanghai/Parts
Chromoproteins
Pigmented compounds that absorb and reflect visible light.
Fluorescent Proteins
Pigmented compounds that absorb UV light and emit visible light.
Luciferase Enzymes
Catalyze a reaction converting luciferin to oxyluciferin. Energy is released in the form of the light.
We created constructs to express 3 different colors of chromoproteins: blue, lime, and yellow. These were taken from the registry, created in 2010 by the Cambridge iGEM team.
BBa_K880005 | promoter/rbs |
BBa_K081005 | promoter/rbs |
We utilized generators to express 2 different colors of fluorescent proteins: RFP and GFP. These parts from the registry.
BBa_J04450 | RFP |
BBa_J04451 | RFP/rbs |
We utilized generators to express 4 different bioluminescent colors: red, green, yellow, and orange.
BBa_K325219 | Red Firefly Luciferase and LRE (under pBAD) |
BBa_K325209 | Green Firefly Luciferase and LRE (under pBAD) |
To create a natural beat generator, we created a light oscillating system. Unfortunately, the time scale was far too long for any practical beat generator (20min per beat). See Fudan 2012 Biowave for more information.