Team:Freiburg/Project/pRIG15 18

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pRIG15_18

Treponama pallidum is responsible for the sexually transmitted disease Syphillis with over 12 million new cases each year worldwide. ¹⁾ For our project we used the sequence of the bacterioferritin protein TpF1, an antigen of Treponema pallidum which reacts specifically with infected human serum. ²⁾ Additionally this antigen shows strong antibody responses what makes it good target for antigen-antibody interaction studies.³⁾

To insert the sequence for TpF1 into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing.

¹⁾ Daskalakis 2008 Syphilis: continuing public health and diagnostic challenges. Current HIV/AIDS reports

²⁾ McGill et al. 2010. Characterization and serologic analysis of the Treponema pallidum proteome. Infection and immunity

³⁾ Jiang et al. 2013 Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis. Clinical and vaccine immunology