Team:Freiburg/Project/pRIG15 8

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pRIG15_8

Infection with the Herpes Simplex Virus 1 leads to life-long persistence of the virus inside the body as a latent infection. ¹⁾ The glycoprotein G is one of 11 glycoproteins expressed by HSV1. ²⁾ The sequence we used for our experiment was obtained from Jaaskelainen et al., 2009. ³⁾

To insert the sequence for HSV1 glycoprotein G into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR (Link zum Labjournal-Eintrag) and then assembled with the digested pSB1C3 backbone using Gibson assembly. To prove correct insertion of our fragment we did a test digest (Link Labjournal) and sent the whole plasmid for sequencing.

¹⁾ Corey et al. 1994. The current trend in genital herpes. Progress in prevention. Sexually transmitted diseases

²⁾ Rekabdar et al. 2002. Dichotomy of glycoprotein g gene in herpes simplex virus type 1 isolates. Journal of clinical microbiology

³⁾ Jaaskelainen et al. 2009. Serological microarray for detection of HSV-1, HSV-2, VZV, and CMV antibodies. Journal of virological methods.