Team:NYU Shanghai/Parts
Chromoproteins
Pigmented compounds that absorb and reflect visible light.
Fluorescent Proteins
Pigmented compounds that absorb UV light and emit visible light.
Luciferase Enzymes
Catalyzes conversion of luciferin to oxyluciferin. Energy is released in the form of the light.
We created constructs to express 3 different colors of chromoproteins: blue, lime, and yellow. These were taken from the registry, created in 2010 by the Cambridge iGEM team.
BBa_K880005 | promoter/rbs |
BBa_K081005 | promoter/rbs |
We utilized generators to express 2 different colors of fluorescent proteins: RFP and GFP. These parts from the registry.
BBa_J04450 | RFP |
BBa_J04451 | RFP/rbs |
We utilized generators to express 4 different bioluminescent colors: red, green, yellow, and orange.
BBa_K325219 | Red Firefly Luciferase and LRE (under pBAD) |
BBa_K325209 | Green Firefly Luciferase and LRE (under pBAD) |
To create a natural beat generator, we created a light oscillating system. Unfortunately, the time scale was far too long for any practical beat generator (20min per beat). See Fudan 2012 Biowave for more information.