Team:EPF Lausanne/Part Collection

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR PART COLLECTION

dCas9-ω

dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.

PAM rich URS J23117

This part is promoter that can be used with our dCas9-ω

This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.






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EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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