Team:Bielefeld-CeBiTec/Software
Our App
Fluorescence Quantification via smartphone
The Problem
Fluorescence is a widely used output signal in many microbiological and biotechnological applications. Green fluorescent protein (GFP) is among the mostly used reporter proteins and has been used in numerous iGEM projects. Nevertheless fluorescence can barely be detected by the eye and quantification turns out to be impossible.
The Solution
We solved this problem with a smartphone App. It determines the median of the greenvalue of some pixels in the sensorspot and compares it to these of the negative control. If the ratio of real sample and negative control is above a certain theshold, the app detects a contamination. It also checks whether the value for the positive control is above a certain value, thereby confirming the functionality of the test. Furthermore it contains specific information regarding the different heavymetals and date rape drugs.