| term | definition |
| 5’UTR | an untranslated region at the 5’ end of the RNA sequence that enhances translation |
| affibody | antibody mimetic, small protein engineered to bind to a large number of target proteins or peptides with high affinity, imitating monoclonal antibodies |
| AIDS | Acquired Immune Deficiency Syndrome, foll-blown diseases after infection with HIV |
| APTES | 3-aminopropyltriethoxysilane, a silane we used to link PDITC to the hydroxyl groups on the plasma activated glass slide of our protein array |
| CFP | cyan fluorescent protein |
| classical cloning | insertion of a DNA fragment for example into a plasmid backbone via restriction enzymes; each fragment has to be inserted separately. For differences to Gibson cloning see here |
| Cy3 | Cyanine 3, fluorescent dye used particularly in biomedical imaging |
| DE3 | nomenclature of E.coli strains, meaning it contains a T7 RNA-polymerase for general protein expression |
| DMSO | Dimethylsulfoxid, added to PCR reactions |
| dNTP | deoxynucleotidetriphosphate, component of DNA and therefore added to PCR reaction mixes |
| Dot Blot | simplification of Northern, Southern or Western Blot in order to save time; proteins are not separated electrophoretically but simply spotted on a membrane and then detected via nucleotide probes (Northern and Southern Blot) or antibodies (Western Blot) |
| ELISA | enzyme-linked immunosorbent assay, antigens are immobilized in a 96-well plate, antibody-antigen-interaction is detected; find more information here |
| fluorophore | fluorescent chemical compound that can emit light upon light excitation; typically contains several combined aromatic groups or plane or cyclic molecules with several π bonds |
| GFP | green fluorescent protein |
| Gibson Assembly | combination of DNA fragments in an isothermal reaction without the need of restriction enzymes; more information and a comparison to classical cloning can be found here |
| glycoprotein | surface protein with oligosaccharide chains covalently bound to the polypeptide; also present on virus surfaces and often provoking an immunogenic response in humans |
| HA-tag | tag derived from the human influenza hemagglutinin and extensively used as a general epitope tag in expression vectors. |
| Her2 protein | biomarker for breast cancer |
| His6 | tag consisting of six histidine residues binding to Ni-NTA and often used for protein purification |
| HIV | Human Immunodeficiency Virus, causes AIDS |
| HSV-1 | Herpes Simplex Virus type 1, also known as Human Herpes Virus type 3 |
| IPTG | Isopropylthiogalactopyranosid, allolactose analogue used to induce the lacI system in cells; often used to induce protein overexpression in bacteria |
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