Team:Freiburg/Home
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Want to use our detection device in your next iGEM project? We built our own simplified and affordable setup. Here you can find a detailed description of how to build and use it.
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In need for expressing tons of protein? We provided a new backbone for protein overexpression meeting all the iGEM standards. We also expanded the iGEM Registry with our
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What does the public think about the DiaCHIP and systems based on synthetic biology? Would people want to use it? Check out the results of our survey.
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Numerous complex processes take place during cell-free expression. Modeling the generation of proteins as well as their diffusion in our system helped us obtaining better results. Get more information here.
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We obtained great results during summer! Our successful measurements of tetanus antibodies in human serum and GFP antibodies binding to cell-free expressed GFP can be found on our results page.
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Modern everyday life is fast...too fast for medical diagnosis relying on huge amounts of time-consuming and costly serological tests. This is why we thought about a fast, universally accessible and affordable diagnostic device.
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Simultaneously screening for hundreds of diseases within a few hours? See how the DiaCHIP achieves this by the revolutionary combination of cell-free expression with an emerging optical method allowing label-free antibody detection.
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Generating a protein array on demand and detecting antigen-antibody interactions in real-time: Find out about our own cell-free expression mix and our specific surface binding system.
Die Texte in den Boxen sind noch ziemlich klein und ziemlich Textwand. Da müssten kürzere, prägnantere Texte mit größerer Schrift und/oder Bild rein. (jb 20150912)
Texte sind zum Überarbeiten. Bilder müssen auch ausgetauscht werden. Länge von 6-7 Zeilen ist optisch am besten. Unter https://2015.igem.org/Team:Freiburg/Testpage3 gibt es eine Variante mit Bildern. Was gefällt euch besser? (sb 20150912)
find ich gut soweit, bin gespannt wie es fertig aussieht (NG)
Abstract
In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference)which is based on a laser detecting the interference of reflecting light from our chip. Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.
