Team:Freiburg/Home

• Home
• Project
• Team
• Results
• Human Practice
• Notebook

• Introduction
• Overview
• Diagnostics today
• The DiaCHIP
  • DNA Engineering
  • Cell-Free Expression
  • Protein Purification
  • Surface Chemistry
  • Optical Detection
• Outlook

• Members
• Acknowledgements
• Partners

• The DiaCHIP
  • Main Results
  • Diagnostics
  • Our Device
  • DNA Array
  • Cell-Free Expression
  • Binding on Surface
  • Modeling
• BioBricks
• Collaborations
• Interlab Study
• Achievements

• Science Fair
• Theater Project
• Public Opinion
• College Talk
• Ethics

• Labjournals
• Techniques
  • Gibson
  • E.coli Strains
• Protocols
• Materials
• Safety
• Wiki-Tricks
• Glossary

• Home
• Project
• Results
• Team
• Practice
• Notebook

Menu

• Own Device
  We built our own simplified and affordable detection device. Check out the step by step instructions!
• New iGEM Backbone
  We provided a new backbone for protein overexpression meeting all the iGEM standards.
  See how we epanded the iGEM registry!
• Human Practice
  What do people think about the DiaCHIP and systems based on synthetic biology?
  Check out the results of our survey and discussions!
• Modeling
  Modeling the generation of proteins during cell-free expression as well as their diffusion in our system helped us obtaining better results. Get more information here.
• Blood Serum Analysis
  We successfully detected tetanus antibodies in human blood serum and measured binding of GFP antibodies to cell-free expressed GFP.
  Visit our results page!
• Diagnostics today
  Nowadays, medical diagnosis is mainly based on huge amounts of time-consuming and costly serological tests. Read more about the issues we tackled!
• The DiaCHIP
  Simultaneously screening for hundreds of diseases within a few hours?
  Find out about the DiaCHIP's potential to revolutionize future diagnostics!
• Our System
  Generating a protein array on demand via cell-free expression and detecting antigen-antibody interactions label-free and in real-time: The principle behind the DiaCHIP!

Einige Überschriften/Texte oben sind irgendwie unscharf. Ansonsten echt gut!! (LS 13/9)
Warum sind bei einigen Überschriften eine Box drum und bei manchen nicht? (ps1309)

DiaCHIP - Enlightening Diagnostics

In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference). Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.