Team:Austin UTexas/Project/Plasmid Study
--this is a first draft--
Spring 2015 Data Set
Plasmid Construction and Transformation
Plasmids were constructed with standard BioBrick cloning procedures and enzymes. Composite plasmids were constructed using the following BioBrick combinations:
| Fluorescent Protein BioBrick | Fluorescent Protein | Promoter/RBS BioBrick | Promoter;RBS Strength |
| BBa_K592100 | BFP | BBa_K608004 | Strong; Weak |
| BBa_K592100 | BFP | BBa_K608007 | Medium; Weak |
| BBa_E0020 | ECFP | BBa_K608007 | Medium; Weak |
| BBa_E0020 | ECFP | BBa_K608003 | Strong; Medium |
| BBa_E0020 | ECFP | BBa_K608002 | Strong; Strong |
| BBa_K592101 | YFP | BBa_K608002 | Strong; Strong |
| BBa_K592101 | YFP | BBa_K608006 | Medium; Medium |
| BBa_K592101 | YFP | BBa_K608007 | Medium; Weak |
| BBa_K864100 | SYFP2 | BBa_K608002 | Strong; Strong |
| BBa_K864100 | SYFP2 | BBa_K314100 | Strong; Very Strong |
| BBa_K864100 | SYFP2 | BBa_K608006 | Medium; Medium |
| BBa_E0030 | EYFP | BBa_K608007 | Medium; Weak |
| BBa_EE030 | EYFP | BBa_K608006 | Medium; Medium |
| BBa_E0030 | EYFP | BBa_K314100 | Strong; Very Strong |
