Team:Freiburg/Project/pRIG15 18

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pRIG15_18

Treponama pallidum is responsible for the sexually transmitted disease Syphillis with over 12 million new cases each year worldwide. ¹⁾ For our project we used the sequence of the bacterioferritin protein TpF1, an antigen of Treponema pallidum which reacts specifically with infected human serum. ²⁾ Additionally, this antigen shows strong antibody responses what makes it good target for antigen-antibody interaction studies.³⁾

Figure 1: pRIG15_18. BBa_K621005 inserted into the submission vector pSB1C3.

To insert the sequence for TpF1 into pSB1C3 we designed Gibson primers with compatible overhangs that also included the start codon ATG. This fragment was amplified via PCR and then assembled with the digested pSB1C3 backbone using Gibson Assembly. To prove correct insertion of the fragment we performed a test digest and verified the part by sequencing.

Get the sequence in genebank format: BBa_K1621005.gb.

¹⁾ Daskalakis 2008 Syphilis: continuing public health and diagnostic challenges. Current HIV/AIDS reports

²⁾ McGill et al. 2010. Characterization and serologic analysis of the Treponema pallidum proteome. Infection and immunity

³⁾ Jiang et al. 2013 Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis. Clinical and vaccine immunology