Team:Freiburg/Protocols/Ligation

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Ligation

amount	Substance
50 ng	Backbone
3fold molar amount	Insert
2 µl	10X T4-Ligase Buffer
1 µl	T4-Ligase
up to 20 µl	H2O

30 min at RT

Equation for insert calculation

Ligation

required Volume of Insert = 3 x Volume(Bb) x length(In) x concentration(Bb) / [ length(Bb) x concentration(In) ]

In short: V(In) = 3 x V(Bb) x l(In) x c(Bb) / [ l(Bb) x c(In) ]

protocols old