Team:Freiburg/Protocols/MesurementGFP
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Measurement of GFP Expression
Terrific Broth
Component | for 1 litre medium | remarks |
---|---|---|
Monopotassium phosphate | 5.6 g | |
Potassium phosphate dibasic | 28.9 g | |
yeast extract | 10 | |
Thiamine | 10 mg | added after sterilisation (filter sterilized) |
Glucose (20%) | 40ml | added after sterilisation (filter sterilized) |
autoclave medium than add filter sterilized Thiamine and Glucose
Store at 4°C