Team:Freiburg/Protocols/MesurementGFP

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Measurement of GFP Expression

material: chemicals, used kits, …
duration: 90 min


Measurement of expressed GFP

  • All fluorescence measurements were performed with a microplate reader in blach plates with transparent bottom
  • As Exitation wavelength 480nm were choosen
  • Emission was measured at 520nm or a full spectrum was taken from 500 to 700 nm
  • Reactions were either performed in a tube and transferred to the microplate after the cell-free reaction or directly performed inside the platereader
  • when the reaction was performed inside the Platreader the wells were covered with foil to avoid evaporation
  • After the reaction and Measurement samples were stored at 4°C for further folding or directly frozen at -20°C