Interlab Requirements

Objective

The iGEM interlab study embodies iGEM’s core values of collaboration, the development of an open community, the education of aspiring young scientists, and the advancement of synthetic biology. Thus, for the 2015 interlab study, the objective is for teams from around the globe to obtain fluorescence data from 3 different genetic devices. This aggregated data will not only aid the detailed characterization of these Biobricks, but also promote further joint scientific efforts of the iGEM community in synthetic biology.

Tested Devices

devices and controls here.

All of our devices were built in the PSB1C3 backbone. The Chassis for all of our devices was E. coli BL21.

Device 1: J23101+I13504 -> PSB1C3

Device 2: J23106+I13504 -> PSB1C3

Device 3: J23117+I13504 -> PSB1C3

Controls

Positive Control: BBa_I20270 -> PSB1C3

Negative Control: BBa_R0040 -> PSB1C3

Construction of the Devices

Details about Construction

Testing the Devices

Details about testing

Protocols

Protocols for construction of devices here.

DNA Kit Plate Instructions (http://parts.igem.org/Help:2015_DNA_Distribution)
Transformation (http://parts.igem.org/Help:Protocols/Transformation)
Preparing overnight cultures for testing
• Prepare culture in a 10 mL culture tube with a loose top
• Add 5mL LB using a seriological pipette
• Add 5uL of 1000x antibiotic (Chloramphenicol.)
• Pick a distinct colony using a pipette tip and eject tip into tube.
Miniprep – using Qiagen protocol
Transformation
Colony PCR (selecting transformants from the transformation plates)

Results

Results over here.

Team:UMaryland/Interlab