Team:Brasil-USP/Notebook/protocols
Protocols
Notebook
Protocols
Table of contents
- Circuit Assembly Protocols
- Calcium chloride transformation with heat shock in Escherichia coli DH5α
- Plasmid extraction
- Digestion of plasmidial DNA
- Ligation reaction (Cohesive ends)
- Agarose Gel Electrophoresis
- Directed mutagenesis PCR (for restriction site elimination) using a plasmid template for restriction site elimination
- PCR amplification (applied to lcp)
- PCR amplification for difficult amplicons (applied to roxA)
- Gibson assembly
- Characterization Protocols
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