Team:Cornell/notebook
- WHAT WE DO
-
fishPHARM
- DRY LAB
- fishBit
- Heimdall Monitoring
- MODELING
- Math Modeling
- Animation
- DISCUSSION
- Policy and Practices
- Entrepreneurship
- Collaborations
- DEVELOPMENT
- Notebook
- Future Applications
- Safety
- OUTREACH
- OUR TEAM
Overview
Week 1
Wetlab
EcnB part extracted from A(ABEF) synthesized plasmids and ligated with H backbone (pSB1C3). Parts were column purified, ligated, then transformed. Had some issues growing cultures with ligations. We are excited to start cloning!
EcnB part extracted from A(ABEF) synthesized plasmids and ligated with H backbone (pSB1C3). Parts were column purified, ligated, then transformed. Had some issues growing cultures with ligations. We are excited to start cloning!
Drylab
Dry lab discussed and finalized a design as well as the dimensions for the fish tag. Parts were designed through Solidworks in member’s own time.
Dry lab discussed and finalized a design as well as the dimensions for the fish tag. Parts were designed through Solidworks in member’s own time.
Outreach
We began searching for fish farm contacts, and started our YOURS mentorship program. We introduced our mentors to the kids and taught them about what DNA is.
We began searching for fish farm contacts, and started our YOURS mentorship program. We introduced our mentors to the kids and taught them about what DNA is.
Week 2
Wetlab
Week one steps were redone. Started gel purifications instead of column purifications, so plasmids were re-cultured (pre-ligation). We had some issues with low DNA concentrations after gel purifications, so we increased amount of DNA used in digestions.
Flavo Subteam
The flavo subteam made cultures of two strains of Flavobacterium, and measured growth of bacteria with spectrophotometer. In addition, daily OD readings were taken to determine growth rate.
Week one steps were redone. Started gel purifications instead of column purifications, so plasmids were re-cultured (pre-ligation). We had some issues with low DNA concentrations after gel purifications, so we increased amount of DNA used in digestions.
Flavo Subteam
The flavo subteam made cultures of two strains of Flavobacterium, and measured growth of bacteria with spectrophotometer. In addition, daily OD readings were taken to determine growth rate.
Drylab
Dry lab continued with the CAD design of the fish tag. We also did research on a list of potentially compatible materials for the fish tag, including tubing, clamps, and syringes. .
Dry lab continued with the CAD design of the fish tag. We also did research on a list of potentially compatible materials for the fish tag, including tubing, clamps, and syringes. .
Outreach
The YOURS kids got a tour of our lab and we taught them more about how DNA works in our body as the determining factor of our genetic characteristics. They were able to place their fingers on plates to “plate bacteria” and we showed them how to incubate the plates to help the bacteria grow. We helped them carry out an experiment that extracted DNA from strawberries and we showed them proper pipetting techniques. We also did a fun experiment with the kids with basic cooking ingredients to make a “volcano explode” to introduce chemical reactions.
We narrowed our search to a few fish hatcheries in the upstate New York region and began contacting them.
The YOURS kids got a tour of our lab and we taught them more about how DNA works in our body as the determining factor of our genetic characteristics. They were able to place their fingers on plates to “plate bacteria” and we showed them how to incubate the plates to help the bacteria grow. We helped them carry out an experiment that extracted DNA from strawberries and we showed them proper pipetting techniques. We also did a fun experiment with the kids with basic cooking ingredients to make a “volcano explode” to introduce chemical reactions.
We narrowed our search to a few fish hatcheries in the upstate New York region and began contacting them.