Team:WHU-Pharm/Experiments
Experiments
Purification of Enzymes
In order to allow cAMP level to be inversely related to glucose concentration, we need to introduce energy coupling pathways into our system. For the purpose of reducing the complexity of the system, we choose to import glycolysis pathway and lactate dehydrogenase (LDH) to convert glucose to lactate, allowing NAD+ to recycle. Besides, we need to include enzymes that catalyze the conversion of AMP to ATP and AMP to cAMP. Therefore, the first task of our project is to purify these enzymes.
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